生脈散對大鼠餵食高脂飲食所造成之胰島素阻抗、TNF-α 與氧化壓力之影響   全文下載

Effect of Shengmai San on Insulin Resistance, Tumor Necrosis Factor-Alpha and Oxidative Stress in Rats Fed on a High-Fat Diet

生脈散是一種著名的中藥複方用來治療冠心病，而胰島素阻抗則是造成冠心病發展與形成最可能的解釋。本研究探討生脈散對高脂飲食所引起之胰島素阻抗、發炎細胞激素的產生及氧化壓力的影響。以雄性Wistar大鼠為實驗動物，分別餵食低脂控制組飲食，高脂飲食以及高脂飲食含4%生脈散共八週，在實驗第七週時進行葡萄糖耐受測試。與單純高油飲食組相比，餵食生脈散飲食之大鼠會明顯降低血槳果糖胺的濃度（p < 0.05）並有降低在給予葡萄糖溶液後血糖及胰島素之曲線下面積的趨勢（p < 0.1）。此外，生脈散具有降低肝臟與心臟中之TnF-α及脂質過氧化程度。但是生脈散並不影響大鼠禁食血漿中血糖、胰島素、三酸甘油酯、游離脂肪酸、高密度脂蛋白膽固醇及胰島素阻抗指標（HOMA）。由我們的結果顯示生脈散可能輕微或不明顯降低胰島素阻抗，但有抗氧化及抗發炎的特性。

Shengmai San (SMS) is a well-known traditional Chinese medicine formula prescribed for patients with coronary heart disease (CHD). Insulin resistance is the most likely explanation for the development and progression of this disease. To investigate the effect of SMS on high-fat diet-induced insulin resistance, inflammatory cytokine production and oxidative stress, male Wistar rats were fed with low-fat control diet, high-fat diet and high-fat diet supplemented with 4% SMS for eight weeks. An oral glucose tolerance test was conducted in the seventh week. Rats fed the SMS diet had significantly lower plasma fructosamine concentration (p < 0.05) and tended to have lower (p < 0.1) AUC values of plasma glucose and insulin concentrations after glucose challenge, compared to those fed the high-fat diet. Moreover, SMS reduced TNF-α and lipid peroxidation levels in the liver and heart. However, SMS had no effect on fasting plasma concentrations of glucose, insulin, triglyceride, non-esterified fatty acids, total cholesterol, HDL-choles-terol and insulin resistance index (homeostasis model assessment; HOMA) in rats. Our results show that SMS may have little or no significant effect on reducing insulin resistance, but display anti-oxidative and anti-inflammatory properties.