以老鼠肝臟細胞株評估杜莎藻（Dunaliella salina）類胡蘿蔔素萃取物抗鎘誘發之細胞毒性和轉形生長因子誘發之平滑肌 α 肌動蛋白表現   全文下載

Evaluation of Carotenoid Extract from Dunaliella salina against Cadmium-Induced Cytotoxicity and Transforming Growth Factor β1 Induced Expression of Smooth Muscle α-Actin with Rat Liver Cell Lines

本實驗對杜莎藻（Dunaliella salina）類胡蘿蔔素萃取物之成分定性定量，並評估其抗鎘（cadmium, Cd）誘發肝細胞毒性和對轉形生長因子（transforming growth factor β1, TGF-β1）誘發肝星狀細胞（hepatic stellate cells, HSCs）活化之影響。All-trans-β-carotene（474.82 mg/g extract）和9- or 9′-cis-β-carotene（425.64 mg/g extract）為此萃取物中主要的類胡蘿蔔素，二者共占萃取物中總類胡蘿蔔素含量的90.30%，其他類胡蘿蔔素為all-trans-lutein (22.77 mg/g extract), all-trans-zeaxanthin (39.26 mg/g extract), 13- or 13′-cis-β-carotene (17.18 mg/g extract), all-trans-α-carotene (9.26 mg/g extract) 和 9- or 9′-cis-α-carotene (8.24 mg/g extract)。對於減少經鎘處理之Clone 9 細胞產生的細胞毒性和TGF-β1處理之 HSC-T6細胞的平滑肌α肌動蛋白表現（smooth muscle α-actin, αSMA）上，此藻類萃取物展現較all-trans-β-carotene高的能力。且藻類胡蘿蔔素萃取物用量與其效力有正相關。"

The carotenoid extract from Dunaliella salina was identified and quantitated. The effects against cadmium (Cd)-induced hepa-totoxicity and transforming growth factor β1 (TGF-β1)-induced activation of hepatic stellate cells (HSCs) were evaluated. All-trans-β-carotene (474.82 mg/g extract) and 9- or 9′-cis-β-carotene (425.64 mg/g extract) were major carotenoids in the extract, accounting for 90.30% of total carotenoids (997.17 mg/g extract). Other carotenoids were all-trans-lutein (22.77 mg/g extract), all-trans-zeaxan-thin (39.26 mg/g extract), 13- or 13′-cis-β-carotene (17.18 mg/g extract), all-trans-α-carotene (9.26 mg/g extract) and 9- or 9′-cis-α-carotene (8.24 mg/g extract). The algal extract had a higher effect (p < 0.05) than all-trans-β-carotene in the reduction of cytotoxic-ity for CdCl2-treated Clone 9 cells and expression of smooth muscle α-actin (αSMA) for TGF-β1-treated HSC-T6 cells. These effects were shown to be concentration-dependent.