HPLC法測定五靈脂藥材中雙黃酮成分的含量   全文下載

Determination of Biflavones from Trogopterus Feces by HPLC

首次以高效液相層析法建立五靈脂藥材中穗花杉雙黃酮和扁柏雙黃酮兩種主要的雙黃酮的含量測定方法。通過層析管柱Alltima C-18（250 × 4.6 mm, 5.0 mm）和流速為1.0 mL/min 的乙?—1% 乙酸溶液梯度洗脫可得到很好的色層分離，採用光二極體陣列檢測器檢測，檢測波長為330 nm。分析物的含量在測定範圍內呈良好的線形（R2 > 0.999），同日內和異日間再現性良好（RSD < 2.40%），分析物回收率分別為103.5% 和97.3%。該方法簡易、準確，研究結果顯示，應用此HPLC 法，可以評估不同產地五靈脂藥材的品質。"

A reversed-phase high performance liquid chromatographic method was developed to simultaneously determine two major biflavones, amentoflavone and hinokiflavone, in Trogopterus Feces. The chromatographic separation was achieved with a linear gradient of acetonitrile in 1% acetic acid at a flow rate of 1.0 mL/min using an Alltima C-18 column (250 × 4.6 mm, 5.0 μm). Detection was carried out using a photodiode array detector at 330 nm. The calibration curves of all analytes showed good linearity over the investigated ranges (R2 > 0.999). Repeatability was evaluated by intra-day and inter-day assays and RSD values were less than 2.40%. The recoveries were 103.5% and 97.3%. This developed method was simple and accurate. The results suggested that the HPLC method could be used to evaluate the quality of Trogopterus Feces from different producing areas.