綠豆皮纖維、米糠纖維與檸檬渣纖維對葡萄糖效應之體外試驗探討   全文下載

In Vitro Interactions on Glucose by Different Fiber Materials Prepared from Mung Bean Hulls, Rice Bran and Lemon Pomace

本研究將綠豆皮、米糠及檸檬渣以化學洗滌法製備膳食纖維，探討膳食纖維之體積密度、保水性、保油性、膨脹性、溶解性等特性，進而探討其對葡萄糖吸附力，及對澱粉?分解能力之影響。結果顯示綠豆皮、米糠和檸檬渣纖維顆粒愈小，體積密度愈大，保水性和保油性愈低。其中綠豆皮纖維有較高的體積密度，保水性顯著高於纖維素，在大顆粒（> 35 mesh）的膨脹性顯著高於纖維素和米糠纖維；米糠纖維則保水性、保油性和膨脹性顯著高於纖維素；檸檬渣纖維的保水性、保油性、膨脹性和其水溶性固形物的溶解度顯著高於其他組。而對葡萄糖吸附效應的體外試驗結果，顯示綠豆皮纖維能夠對低濃度之葡萄糖有較高的吸附力，米糠和檸檬渣纖維則在高濃度葡萄糖下有顯著較佳的吸附效果；對澱粉?活性之抑制效果，顯示小顆粒（<50 mesh）檸檬渣纖維的效果最佳。另外模擬腸胃道對葡萄糖吸收的試驗，顯示在 180 分鐘 30~50 mesh 的綠豆皮纖維之葡萄糖擴散速率有顯著性的降低。以上纖維對葡萄糖的吸附能力影響，可能減少腸道中可利用的葡萄糖量，而降低餐後血糖濃度。

Fiber was prepared from mung bean hulls, rice bran and lemon pomace by the chemical precipitation method and then characterized. The characteristics investigated included bulk density, water-holding capacity, oil-holding capacity, swelling properties, water solubility and glucose adsorption and diffusion in vitro experiments. The results showed that the smaller the fiber particles were, the higher the bulk density was and the lower the ability of the fiber was to absorb water and oil. Mung bean hull fiber had a significantly higher bulk density and water-holding capacity than cellulose. The swelling property of mung bean hull fiber was also significantly higher than that of cellulose. The water-holding capacity, oil-holding capacity and swelling property of rice bran fiber were all significantly higher than those values for cellulose. The water-holding capacity, oil-holding capacity, swelling property and water solubility of lemon pomace fibers were also significantly higher than those for the other experimental groups. The glucose effects in the in vitro experiments indicated that mung bean hull fiber had a higher level of adsorption when the glucose level was low. Fiber made from rice bran and lemon pomace had much better adsorption when exposed to high levels of glucose. In a mixed system including fiber, amylase and substrate, the level of glucose produced in the mixture containing lemon pomace fiber with a size <50 mesh was the lowest, and it was the best inhibitor of amylase activity. After 180 min of glucose adsorption, mung bean hull fiber with a size of 30-50 mesh was able to lower glucose diffusion. All of these mechanisms might contribute the rate reduction of glucose adsorption in the intestines, as a result, decrease the postprandial serum glucose concentration.