陽春砂及 10 種山薑屬植物 nr DNA 的 ITS1 序列分析   全文下載

Sequence Analysis Based on ITS1 Region of Nuclear Ribosomal DNA of Amomum villosum and Ten Species of Alpinia

陽春砂Amomum villosum Lour. 的成熟果實是常用中藥材砂仁的來源。市場調查發現山薑屬（Alpinia Roxb.）一些植物的果實常用作砂仁的代用品或偽品，這些果實在形態和組織學上與砂仁很相似，給鑒別帶來一定困難。本研究測定了陽春砂及用作砂仁偽品的10 種山薑屬植物的核糖體DNA ITS1 序列，結果表明陽春砂和10 種山薑屬植物的序列長度很相近，為175~179 bp，序列中G+C 含量為53.37~56.74%。通過排序比較發現，陽春砂和10 種山薑屬植物的ITS1 序列中共有55 個變異位點。10 種山薑屬植物序列之間的鹼基替代率為0.57~9.60%，但陽春砂和這10 種山薑屬植物之間的差異則高達17.61~21.59%。這些分子水平上的證據表明核糖體DNA ITS1序列可用于建立區別陽春砂及來源於山薑屬植物之砂仁偽品的方法。

Ripe fruits of Amomum villosum Lour. are important traditional Chinese medicines and food spices. Due to similar morphological and anatomical characteristics, fruits from some Alpinia species are used clinically as substitutes or adulterants of Fructus Amomi. In this study, we determined the nrDNA ITS1 (internal transcribed space 1) sequences from Amomum villosum and ten Alpinia species, which are adulterants of Fructus Amomi. The ITS1 sequences of these species were 175~179 bp in length, and the G+C contents were 53.37~56.74%. Sequence alignment revealed that there were 55 polymorphic nucleotide sites among the tested species. The base substitution were 0.57~9.60% within the ten Aplinia species, but 17.61~21.59% between Amomum villosum and the Alpinia species. These molecular data indicated that ITS1 sequences can be used to differentiate Fructus Amomi and its substitutes or adulterants from various Alpinia species.