月旦知識庫
 
  1. 熱門:
 
首頁 臺灣期刊   法律   公行政治   醫事相關   財經   社會學   教育   其他 大陸期刊   核心   重要期刊 DOI文章
JOURNAL OF FOOD AND DRUG ANALYSIS 本站僅提供期刊文獻檢索。
  【月旦知識庫】是否收錄該篇全文,敬請【登入】查詢為準。
最新【購點活動】


篇名
香茹分離所得 dehydrocostus lactone 誘發 A549 肺腺癌細胞凋亡之研究   全文下載 全文下載
並列篇名
Induction of Apoptosis of A549 Lung Cancer Cell Line by Dehydrocostus Lactone Isolated from Glossogyne Tenuifolia
作者 許夏芬 (Hsia-Fen Hsu)吳永昌陳麗琴 (Li-Chin Chen)洪哲穎 (Jer-Yiing Houng)
中文摘要
香茹(Glossogyne tenuifolia)為台灣傳統使用於消暑、解熱與保肝之草藥。本研究由香茹粗萃取物中分離出兩個純化合物,包括去氫木香內酯(DL, 6-hydroxy-guaia-4(15), 10(14), 11(13)-trien-12-oic acid lactone)與穿木香內酯(ML, 4(15), 10(14)-guaiadien-12,6-olide lactone)。此二化合物之化學結構係以光學方法來鑑定,並被確認為首次由香茹分離出之物質。由細胞毒殺試驗結果發現,DL 在抑制人類肺腺癌A549 細胞生長的效應上,具有時間與劑量之倚賴關係,但ML 對A549 細胞生長的抑制效果則不顯著。由流式細胞儀分析結果顯示,經DL 處理後A549 細胞之生長會停滯於sub-G1 期。此外,由caspase-9 與-3 酵素之活化現象可進一步證實DL 會使A549 細胞經由凋亡路徑(apoptosis pathway)使癌細胞死亡。除了A549 細胞外,DL 對乳癌細胞MDA-MB-231 與肝癌細胞Hep G2 亦具有極強的毒殺效果。雖然DL 是一個文獻記載已知的化合物,然而本論文應為第一個探討其對A549 肺腺癌細胞毒性的研究報告。"
英文摘要
Glossogyne tenuifolia is a traditional antipyretic and hepatoprotective herb used in Taiwan. In this study, dehydrocostus lactone (DL, 6-hydroxy-guaia-4(15), 10(14), 11(13)-trien-12-oic acid lactone) and mokko lactone (ML, 4(15), 10(14)-guaiadien-12,6-olide lactone) were isolated from the crude extract of G. tenuifolia. Structures of these two compounds were elucidated by spectroscopic evidence. These two sesquiterpene lactones were identified for the first time from G. tenuifolia. From cytotoxicity tests, DL inhibited the proliferation of human lung cancer A549 cells in a time- and dose-dependent manner, whereas the cytotoxicity of ML on A549 cells was unimportant. The cytometric analysis showed that the A549 cells were arrested at the sub-G1 phase by the treatment of DL. The evidence of the activation of caspase-9 and -3 verified that the death of A549 cells was through the apoptosis pathway. Other than A549 cells, DL also exhibited strong cytotoxicity on MDA-MB-231 (breast cancer) and Hep G2 (hepatoma) cells. Although DL is a known compound, to our knowledge, this work is the first to investigate its cytotoxicity on A549 cells.
起訖頁 107-115
關鍵詞 去氫木香內酯穿木香內酯香茹細胞毒性細胞凋亡A549肺腺癌細胞dehydrocostus lactonemokko lactoneGlossogyne tenuifoliacytotoxicityapoptosislung cancer cell line A549
刊名 JOURNAL OF FOOD AND DRUG ANALYSIS  
期數 200904 (17:2期)
出版單位 衛生福利部食品藥物管理署
該期刊-上一篇 以寧海都寧配合分光光度法檢測藥品中利欣諾普之含量
該期刊-下一篇 採集時間對基隆山藥(Dioscorea pseudojaponica Yamamoto)中皂X之影響
 

新書閱讀



最新影音


優惠活動




讀者服務專線:+886-2-23756688 傳真:+886-2-23318496
地址:臺北市館前路28 號 7 樓 客服信箱
Copyright © 元照出版 All rights reserved. 版權所有,禁止轉貼節錄