高效液相色譜法測定白花蛇舌草及其代用品中齊墩果酸和熊果酸的含量   全文下載

Determination of Oleanolic Acid and Ursolic Acid in Oldenlandia diffusa and Its Substitute Using High Performance Liquid Chromatography

本文建立一種改良高效液相色譜法用於測定白花蛇舌草及其代用品中齊墩果酸和熊果酸的含量。通過優化流動相條件，齊墩果酸和熊果酸得到完全分離。同時提取條件也被優化。系統的市場觀察顯示傘房花耳草常被用作白花蛇舌草。通過對二十七批白花蛇舌草和十七批傘房花耳草的分析發現白花蛇舌草中齊墩果酸和熊果酸的含量低於傘房花耳草。齊墩果酸和熊果酸的含量在冷凍乾燥與低溫烘乾的樣品中無明顯差異。該測定方法有助於白花蛇舌草和其代用品的品種鑑定以及質量評價。

An improved quality evaluation method for Oldenlandia diffusa (Willd.) Roxb. and its substitute was established by a welldefined high performance liquid chromatographic (HPLC) method. Firstly, a complete separation of oleanolic acid and ursolic acid was successfully achieved by optimizing the mobile phase systems based on previous reports. The extraction method was optimized and the less toxic solvent was selected for extraction. The systematic survey of herbal markets has shown that O. corymbosa (L.) Lam are widely available and used indiscriminately as O. diffusa. Twenty-seven samples of O. diffusa and seventeen samples of O. corymbosa were analyzed. The results showed that the contents of oleanolic acid and ursolic acid in O. diffusa were generally lower by almost two times than those in O. corymbosa. Moreover, the contents of oleanolic acid and ursolic acid between the fresh and dry samples were compared. The introduction of quality evaluation method could be useful for the species identity and quality evaluation of O. diffusa and its substitute.