以三氯異三聚氰酸及二氯異氰酸鈉氧化Luminol為基礎之流動注入分析化學發光系統檢測異菸鹼異丙醯胼   全文下載

Determination of Iproniazid by Flow Injection Analysis with Chemiluminescence Detection Based on the Oxidation of Luminol by Trichloroisocyanuric Acid and Sodium Dichloroisocyanurate

本研究係以簡單、快速及準確的化學發光法分析異菸鹼異丙醯?（iproniazid）， 方法依據三氯異三聚氰酸（TCCA） 及二氯異氰酸鈉（SDCC） 在鹼性介質中氧化Luminol 所產生之化學冷光（chemiluminescence, CL）反應。反應過程中發現異菸鹼異丙醯?能大幅增加化學冷光的強度。異菸鹼異丙醯?在TCCA-luminol 及SDCC-luminol 系統之最低檢測限量（3σ）分別是8.0 and 76 ppb。在TCCA-luminol 系統，化學冷光強度與異菸鹼異丙醯?濃度介於50-1000 ppb 及1000-2000 ppb 之間呈直線相關，在SDCC-luminol 系統，化學冷光強度與異菸鹼異丙醯?濃度介於100-2000 ppb 之間呈直線相關。應用本方法分析合成物或人體體液中的異菸鹼異丙醯?都具有可接受的精準性。"

A simple, rapid and accurate chemiluminescent method is described for the determination of iproniazid. The proposed method is based on the chemiluminescence (CL) generated during the oxidation of luminol by trichloroisocyanuric acid (TCCA) and sodium dichloroisocyanurate (SDCC) in alkaline medium. It was found that iproniazid could greatly enhance the CL intensity of the mentioned reactions. The detection limits (3σ) were 8.0 and 76 ppb of iproniazid for the TCCA-luminol and SDCC-luminol systems, respectively. The relative CL intensity was linear with the iproniazid concentration in the range of 50-1000 and 1000-2000 ppb for TCCA-luminol system, and 100-2000 ppb for SDCC-luminol system. The method was applied to the determination of iproniazid in a synthetic sample as well as in human serum with acceptable accuracy and precision.