利用 HPLC 配合後柱衍生反應及矽酸鎂管柱沖提後螢光偵測分析蜂蜜中 Aldicarb、Propoxur、Carbofuran、Carbaryl 及 Methiocarb 之農藥殘留   全文下載

Determination of Aldicarb, Propoxur, Carbofuran, Carbaryl and Methiocarb Residues in Honey by HPLC with Post-column Derivatization and Fluorescence Detection after Elution from a Florisil Column

本研究目的在開發一種快速分析方法， 其利用HPLC 配合後柱衍生反應及螢光偵測（HPLC/PCD-FL） 分析蜂蜜中aldicarb、propoxur、carbofuran、carbaryl 及methiocarb 殘留。矽酸鎂充填管柱用於樣品製備，顯示良好的效果。不同標準品添加量（50、100、200 ng/g）下，回收率介於72.02% 及92.02% 之間。檢測極限視不同含氮甲基胺基甲酸鹽農藥而落在4–5 ng/g。本研究的萃取方法簡單、便宜又快速。因此，此法可被有效地應用在常態監測之研究。

The aim of this study was to develop an easy analytical method for determining aldicarb, propoxur, carbofuran, carbaryl and methiocarb residues in honey by high performance liquid chromatography with post-column derivatization and fluorescence detection (HPLC/PCD-FL) for routine analysis. A florisil packed column was used in sample preparation step and showed good performance. The recoveries ranged between 72.02 and 92.02% for various spiking levels (50, 100, 200 ng/g). The limits of detection were 4–5 ng/g, depending on different N-methylcarbamate pesticides. The extraction method used in the present study was easy, inexpensive, and rapid. Thus, it could be used efficiently in the routine monitoring studies.