Rubus coreanus Miquel 萃取物對小鼠之類造骨MC3T3-E1 細胞增殖與分化的影響   全文下載

Effects of Rubus coreanus Miquel Extracts on the Proliferation and Differentiation of Mouse Osteoblast-like MC3T3-E1 Cells

Rubus coreanus Miquel（RCM）是一種可食的紅莓，生長於韓國與中國野外，其未成熟果實已被民間用來治療陽萎並作為利尿劑。本篇研究的目的在於調查RCM對小鼠之類造骨MC3T3-E1細胞增殖與分化的影響。本研究中，RCM萃取物（1-10 μg/mL）可使造骨細胞生存力增加。RCM萃取物也會增加鹼性磷酸?（ALP）活性及MC3T3-E1細胞之骨鈣蛋白質分泌（顯著發生於1-10 μg/mL）。尤其在濃度10 μg/mL時，ALT活性約增為對照組的4.3倍。Alizarin red染色法及骨鈣蛋白質分泌分析法顯示，藉由誘發礦化作用的活化，RCM萃取物可刺激造骨細胞之分化。此外，反轉錄聚合?鏈反應及西方點墨分析顯示，RCM萃取物在mRNA與蛋白質的層級間誘發活化。本結果認為RCM萃取物藉由促進類造骨MC3T3-E1細胞之增殖與分化而使造骨細胞功能更為彰顯。

Rubus coreanus Miquel (RCM), one a type of edible red raspberry, grows wild in Korea and China and its unripe fruit has been used as a folk medicine for the treatment of impotence and as a diuretic agent. The goal of this study was to investigate the effects of RCM on the proliferation and differentiation of mouse osteoblast-like MC3T3-E1 cells. In this study, RCM extract (1-10 μg/mL) enhanced the viability of osteoblast cell. RCM extract also increased alkaline phosphatase (ALP) activity and osteocalcin secretion of MC3T3-E1 cells (significant at 1-10 μg/mL). Especially, at a concentration of 10 μg/mL ALP activity was increased by approximately 4.3 times in comparison with the control. Alizarin red staining and osteocalcin secretion assay showed that RCM extract stimulates differentiation of osteoblast cells by inducing the activation of mineralization. Moreover, reverse transcriptase polymerase chain reaction and western blotting assay indicated that RCM extract induced the differentiation at mRNA and protein level. These results suggest that RCM extract enhanced the osteoblast function by increasing the proliferation and differentiation of osteoblast like MC3T3-E1 cells.