亞硫酸鹽處理金針花對巨噬細胞生成一氧化氮及DNA損傷之影響   全文下載

Effect of Sulfite-treated Daylily (Hemerocallis fulva L.) Flower on the Production of Nitric Oxide and DNA Damage in Macrophages

本研究為探討不同來源之新鮮金針花與乾燥金針花抑制一氧化氮生成的能力，以及評估二氧化硫的存在是否會影響乾燥金針花抑制一氧化氮的生成及細胞DNA損傷方面的表現。結果顯示，亞硫酸鹽處理及未處理之乾燥金針花清除sodium nitroprusside （SNP） 生成一氧化氮的能力較佳，而新鮮金針花則較差。另外，新鮮金針花與未經亞硫設鹽處理之乾燥金針花抑制lipopolysaccharide （LPS） 誘導巨噬細胞生成一氧化氮的能力較佳，而經亞硫酸鹽處理之乾燥金針花則較差。進一步測定其二氧化硫含量，並換算成相對濃度之亞硫酸氫鈉（NaHSO3），來探討NaHSO3抑制一氧化氮生成及細胞基因傷害的能力。結果顯示，隨著亞硫酸氫鈉濃度的增加，其清除SNP生成一氧化氮的能力隨之增加，且其亦有抑制LPS誘導細胞生成一氧化氮的能力，然抑制能力不高。此外，NaHSO3亦會稍微引起細胞DNA損傷。雖然在加熟時二氧化硫會揮發掉，但仍有二氧化硫錢留之虞，因此對於乾燥金針花的攝取仍應以適量為宜。

The inhibitory effect of fresh or dried daylilies extract on the generation of nitric oxide, and the effects of sulfur dioxide in dried daylily on nitric oxide production and DNA damage in Raw 264.7 macrophages were examined. The extracts from sulfitetreated and untreated dried daylily flowers exhibited stronger scavenging effects on nitric oxide production by sodium nitroprusside (SNP) than that of fresh flower. Fresh and untreated dried daylily flowers exhibited strong inhibitory effect on nitric oxide induced by lipopolysaccharide (LPS) in macrophages, but the sulfite-treated dried daylily did not. The scavenging effect of sulfite on nitric oxide production by SNP was dose-dependent, and sulfite also exhibited low inhibitory effect on nitric oxide induced by LPS in macrophages. Sodium hydrogen sulfite also slightly induced DNA damage in macrophages. Although sulfur dioxide in dried flowers is volatilized during heating, its residual adverse effects should still be concerned.