批式製備具AcE活性抑制之卵蛋白胜肽   全文下載

Protein Hydrolysate Batch Production with Angiotensin I-Converting Enzyme Inhibitory Activity from Egg Whites

運用thermolysin以製備卵蛋白胜?，經定性並分區以探討各區對ACE活性之抑制，並應用自發性高血壓大鼠（SHR）測試卵蛋白胜?的抗高血壓功效。在thermolysin最適條件（pH 8及60°C），及[E]/[S]＝0.02%下，1%粗卵蛋白溶液經水解4小時，離心後澄清液中之產物回收率約80%，此水解物有高度ACE抑制活性（IC50＝33 μg/mL），不具苦味亦無蛋清之腥味，依序經分子量10,000，3,000和1,000 Da膜之超過濾，可提高其濾液對ACE之抑制活性達IC50＝17 μg/mL。Thermolysin可回收重複使用四次而不降低水解物之ACE活性抑制，SHR實驗顯示口服0.2 g/Kg體重能有效降低高血壓。"

Peptides derived from egg whites by thermolysin digestion were fractionated and characterized to investigate their inhibitory activity against angiotensin I-converting enzyme (ACE). The antihypertensive effect of egg white hydrolysates in strain SHR spontaneously hypertensive rats was also investigated. At optimal conditions, pH 8, 60°C, and (E)/(S) = 0.02%, digestion of 1% crude egg white solution by thermolysin was carried for 4 hours. The recovery yield from the supernatant after centrifugation was around 80%. The hydrolysate showed high ACE inhibitory activity (IC50 = 33 μg/mL) and imparted neither bitter taste nor the iron odor of egg whites. Sequential ultra-filtration of hydrolysate with MW cut-off 10,000, 3,000 and 1,000 Da resulted in increased activity from each filtrate up to IC50 = 17 μg /mL. Thermolysin was recycled from the hydrolysate for subsequent batch use for a total of four batches without reduction in anti-hypertensive activity. The hydrolysate demonstrated an anti-hypertensive activity in spontaneously hypertensive rats at an orally administrated dosage of 0.2 g/kg body weight.