利用CHEMOMETRIC HPLC方法同時測定市售品中食用黃色四號及食用紅色四十號   全文下載

Simultaneous Determination of Tartrazine and Allura Red in Commercial Preparation by Chemometric HPLC Method

利用多變校正（multivariate calibrations）及HPLC偵測雙合物（binary mixtures）及市售品（commercial preparation）中之食用黃色四號（TARTRAZINE, TAR）及食用紅色四十號（ALLURA RED, ALL）濃度。於本研究中，以多種波長HPLC數據所得之partial least squares （PLS）、principal component regression （PCR） 及classical least squares （CLS） 以多變校正處理得到CPLS, CPCR, CCLS，層析多變校正（chromatographic multivariate calibrations）係由多種波長波峰面積（x）與濃度（y）的關係可得，多種波長層析及波峰面積數據則產自光電二極體陣列偵測器（photodiode array detector, PDA），本研究使用Watee Symmetry R C185μm 4.6 x 250 mm管柱，移動相為0.2M醋酸緩衝液（pH = 5） 與乙晴 + 甲醇 + 二次蒸餾水（55:20:10, v/v） 之混合，流速1.9 mL/min，在含內標準品（internal standard, IS）食用黃色五號（sunset yellow）情況下，上述設備及條件使得TAR及ALL分離良好；利用標準添加法，本法在測試數種不同合成混合物（synthetic mixtures）上已完成確效，對於市售含TAR及ALL之飲料粉亦可應用本法分析，文內亦顯示本法所得結果間及與單波長HPLC之比較。"

The concentrations of tartrazine (TAR) and allura red (ALL) in binary mixtures and commercial preparation were determined by the combined use of chemometric (or multivariate) calibrations and HPLC method. In this study, partial least squares (PLS), principal component regression (PCR) and classical least squares (CLS) based on multiwavelength HPLC data were refined as multivariate calibration techniques such as CPLS, CPCR, CCLS. The relation between multiwavelength peak area data (x-block) and concentration set (y-block) were used to obtain the chromatographic multivariate calibrations. Multiwavelength-chromatograms or multiwavelength peak area data were obtained by using photodiode array (PDA) detectors. Waters Symmetry® C18 Column 5 μm 4.6 × 250 mm and a combination of 0.2 M acetate buffer (pH = 5), acetonitrile, methanol and bidistilled water (55:20:10 v/v) at the flow rate of 1.9 mL/min were used to obtain a good chromatographic separation between TAR and ALL in presence of sunset yellow (Internal Standard (IS)). These chromatographic multivariate techniques were validated by analyzing the different synthetic mixtures and by using standard addition technique. These methods were applied to the commercial soft drinking powder samples containing TAR and ALL. The results from these chromatographic multivariate techniques were compared with each other as well as obtained by alternative single HPLC method.