大白鼠去卵巢後加味逍遙散對其骨質流失之研究   全文下載

Effects of Chia-wei-hsiao-yao-san on Bone Loss in Ovariectomized Rats

為了探討加味逍遙散（Chia-wei-hsiao-yao-san）、龜鹿二仙膠（Kuei-luerh-hsien-chiao）與雌性素（17-β-estradiol）對於停經後老鼠之骨質疏鬆症的預防效果，我們將Long-Evans老鼠隨機分成五組：第一組老鼠只開刀未去卵巢，每日餵以食用水；第二組老鼠去卵巢後，每日餵以食用水；第三組老鼠去卵巢後，每日餵以龜鹿二仙膠（2.5 g/kg）；第四組老鼠去卵巢後，每日餵以加味逍遙散（2.5 g/kg）；第五組老鼠去卵巢後，同時於背部皮下包埋一支silicon的小管，管內含有17 β-estradiol（17 β-estradiol與膽固醇以1比9之比例混合），模擬皮下注射的方式，調整其劑量使每日平均釋出17 β-estrodiol為10 μg/kg。八週後，發現第四組之骨質密度顯著高於第二組之骨質密度（p < 0.05），而第五組之骨質密度也顯著高於第二組之骨質密度（p <0.01）。在組織切片上可看到在第一組、第四組及第五組老鼠之脛骨的縱切面，其生長板較緻密，但第一組老鼠之脛骨的生長板較第四組及第五組老鼠之脛骨的生長板較緻密。從第四組及第五組老鼠之脛骨的橫切面來看，其mucopolysaccharide的積聚較第三組老鼠少。此外，第一組及第五組之血中鹼性磷酸顯著低於第二組。然而，第五組老鼠之尿液Dpd值較其他組別的老鼠為高，但五組老鼠之尿液NTx數值並無顯著差異。由本實驗結果得知，龜鹿二仙膠對於去卵巢老鼠之骨質疏鬆的預防效果較差，而雌性素及加味逍遙散可有效預防停經後骨質疏鬆的發生，雌性素的效果優於加味逍遙散。雖然如此，但加味逍遙散較不易引起月經紊亂、不正常出血等副作用，效果雖不似雌性素佳，但是有預防骨質疏鬆的效果，仍不失為可替代雌性素改善更年期所造成的骨質疏鬆症的另一選擇。

Preventive effects of two traditional Chinese medicines, Chia-wei-hsiao-yao-san and Kuei-lu-erh-hsien-chiao, on the bone loss induced by ovariectomy in Long-Evans rats were investigated. The rats were divided randomly into five groups. The first group was given a sham operation with administration of distilled water, whereas the second, third and fourth groups were ovariectomized with administration of distilled water, Kuei-lu-erh-hsien-chiao and Chia-wei-hsiao-yao-san, respectively. The rats of the fifth group were embedded by intraperitoneal with a silicon tube containing 17 β-estradiol (10% in cholesterol) at a dose of 10 μg/kg. Eight weeks later, the mean differences of spinal bone mineral density (BMD) in groups 1, 4 and 5 were significantly higher than that in group 2. The mean differences of plasma alkaline phosphatase (ALP) in groups 1 and 5 were significantly lower than those in group 2, but there were no significant differences between groups 1 and 5. In histologic analysis, the cross sections from lower third of the tibial shaft in group 3 showed more mucopolysaccharide accumulation than those in groups 4 and 5. The urinary N-telopeptide of type I collagen (NTx) levels did not differ significantly among groups 1, 2, 3, 4 and 5. Nevertheless, the mean differences of urinary deoxypyridinoline (Dpd) levels in group 5 were significantly higher than those in group 2. 17 β-estradiol and Chia-wei-hsiao-yao-san showed more profound effect than Kuei-lu-erh-hsien-chiao in the prevention of bone loss in the ovariectomized (OVX) rats. Although 17 β-estradiol exerted more profound effect, Chia-wei-hsiao-yao-san showed good compliance and safety for relief of climacteric symptoms for postmenopausal women in a previous study. Therefore, Chia-wei-hsiao-yao-san might be an alternative for preventing bone loss.