以吸附剝除伏特安培法檢測氟奎諾酮類藥物   全文下載

Adsorptive Stripping Voltammetric Determination of Fluoroquinolones in Pharmaceuticals

靈敏的陰極吸附剝除伏特安培法已被開發應用於兩種氟奎諾酮類抗菌藥物—norfloxacin及enoxacin之檢測。二甲基甲醯胺與0.1 M鹽酸分別為norfloxacin及enoxacin之溶劑。氯化鉀為電解質。於-1.02至-1.13 V及0.93至-1.07 V範圍分別偵測norfloxacin及enoxacin之衰減波。Norfloxacin及enoxacin之線性校正範圍與偵測極限分別為20-100 μg/mL及1-40 μg/mL與10 μg/mL及50 ng/mL。另外，10 μg/mL norfloxacin及enoxacin（n = 5）之相對標準偏差分別為1.1%及0.2%。葡萄糖、乳糖、山梨醇、阿拉伯膠、澱粉、硬脂酸鎂及對羥基苯甲酸丙酯不會影響檢測結果。本方法應用於製藥分析上之定量回收相對偏差為1.6-4.75%。

Sensitive cathodic stripping voltammetric methods have been developed for two fluoroquinolone antibacterial drugs, norfloxacin and enoxacin. Dimethylformamide (DMF) and 0.1 M hydrochloric acid were used as media for norfloxacin and enoxacin respectively. Potassium chloride was used as base electrolyte. A reduction wave was observed for norfloxacin within the range of -1.02 to -1.13 V and for enoxacin within -0.93 to -1.07 V. Linear calibration ranges for norfloxacin and enoxacin were observed within 20-100 μg/mL and 1-40 μg/mL with detection limits of 10 μg/mL and 50 ng/mL respectively. Relative standard deviations (RSD) for the analysis of 10 μg/mL norfloxacin and 10 μg/mL enoxacin (n = 5) were observed 1.1% and 0.2%. The presence of glucose, lactose, sorbitol, gum arabic, starch, magnesium stearate, methylparaben and propylparaben did not affect the determination. The methods were used for the analysis of pharmaceutical preparations and quantitative recoveries were obtained with relative deviation within 1.6-4.75%.