膳食類黃酮可抑制3T3-L1脂肪細胞的分化   全文下載

Dietary Flavonoids Suppress Adipogenesis in 3T3-L1 Preadipocytes

本研究探討膳食中攝取的類黃酮物質，包括兒茶素（catechin）、檞黃素（quercetin）、堪非黃酮醇（kaempferol），對3T3-L1脂肪細胞分化的影響。以類黃酮樣本與MDL分化誘導養液共同處理3T3-L1細胞後第八天，發現類黃酮處理之3T3-L1細胞內的三酸甘油脂累積量顯著低於對照組（P < 0.05），兒茶素、檞黃素與堪非黃酮醇（25-100 μM）分別可減少3T3-L1細胞的胞內三酸甘油脂累積量達54.4%，45.7%與29.9%，同時此抑制作用具劑量效應。此外，利用RT-PCR分析，發現兒茶素、檞黃素與堪非黃酮醇均顯著降低3T3-L1細胞中， 與脂肪細胞分化相關的C/EBP-α, APPAR-γ 與SREBP-1三種轉錄因子之mRNA表現量，顯示類黃酮兒茶素、檞黃素與堪非黃酮醇可透過降低脂肪細胞分化相關的轉錄因子表現，抑制脂肪細胞的分化，故可能有助於肥胖的預防。"

This study examined how dietary flavonoids, including catechin, quercetin and kaempferol, affect the differentiation of 3T3-L1 adipocytes. Eight days following induction for differentiation with MDI-medium and simultaneously with the tested flavonoids (day 8), our study found significantly reduced (p < 0.05) intracellular triacylglycerol accumulations of 3T3-L1 cells. This compared positively against the smaller reduction in accumulation found in the vehicle control. This suppressing effect was found to be dose-dependent, and a 54.4%, 45.7% and 29.9% decrease of triacylglycerol production was observed for catechin, quercetin and kaempferol (25-100 μM), respectively. Additionally, RT-PCR assays demonstrated that catechin, quercetin and kaempferol markedly reduced the mRNA expressions of C/EBP-α, PPAR-γ and SREBP-1, which are key transcription-factors for 3T3-L1 adipogenesis. These results suggest that dietary flavonoids suppress 3T3-L1 differentiation by down-regulating adipogenic transcription-factors. Further study recommended to explore the potential health benefits of catechin, quercetin and kaempferol in the control of body weight.