液相層析法對萬應膏貼劑中十種成分之同時分析研究   全文下載

Simultaneous Analysis of Ten Components in Patch Preparation of Wan-Yin-Gau by High Performance Liquid Chromatography

使用逆相HPLC模式進行萬應膏貼劑之成分分析，開發出包含肉桂中cinnamic acid、cinnamaldehyde；白芷及羌活中isoimperatorin；當歸中ferulic acid、芍藥中paeoniflorin；甘草中glycyrrhizin；元參中harpagoside及大黃中emodin, sennoside A, sennoside B等十種指標成分之多成分同時定量分析方法。同時以水性基劑及油性基劑等不同製程，探討對萬應膏品質之影響。萬應膏之試料通過保持在30℃恒溫之HPLC層析管（Inertsil ODS-2, 4.6 mm i.d.×250 mm），移動相採用20%及70% acetonitrile之混合溶液，進行梯度沖提法，以1.0 mL/min之流速沖提。七種指標成分之檢測使用UV偵測器，偵測波長設定250 nm。本法之回收率，同日間及異日間的變異係數均在5%以下，回收率在96.29~103.46%之間。這個分析法對於萬應膏製劑中十種指標成分是安定且值得信賴之定量法。"

A facile HPLC method for the reduction and quantitative measurement of ten marker substances, the active ingredients in formula preparation of Wan-Yin-Gau, was established under the gradient elution in the reversed-phase mode. These marker substances included cinnamic acid, cinnamaldehyde (Cinnamomi Cortex), isoimperatorin (Angelicae Dahuricae Radix and Notopterygii Rhizoma), ferulic acid (Angelicae Sinensis Radix), paeoniflorin (Paeoniae Radix), glycyrrhizin (Glycyrrhizae Radix), harpagoside (Scrophulariae Radix), emodin, sennoside A, and sennoside B (Rhei Rhizoma). The ingredients in the water-based and oil-based patches from different manufactures were also analyzed for quality evaluation. Extracted samples were analyzed with reverse-phase column (Inertsil 5 ODS-2, 4.6 i.d. × 250 mm) at 30°C, eluted with a mixture of 20% and 70% acetonitrile aqueous solution in gradient manner at a flow rate of 1.0 mL/min, and detected at 250 nm. Relative coefficients of variation of intra- and inter-day analysis were less than 5%. All the recoveries were 96.29~103.46%. This method could be applied for the simultaneous determination of ten marker substances in “Wan-Yin-Gau”.