以毛細管電泳分析蜂膠中的類黃酮物質   全文下載

Analysis of Flavonoids in Propolis by Capillary Electrophoresis

本研究以毛細管電泳法分析九種原膠及九種蜂膠商品中的類黃酮含量，首先選擇apigenin 、chrysin 、luteolin 、galangin 、kaempferol 、morin 、myricetin 、quercetin 、quercitrin 、rutin 、hesperetin 、naringin 、naringenin 、daidzein 及genistein 等15 種市售類黃酮作為標準品，以尋求最適分析條件，結果顯示搭配毛細管區帶電泳（CZE）及膠束動力學毛細管層析（MECC）兩種分離模式，可完全分離此15 種類黃酮標準品。最適當的CZE分析條件是在25 ℃下，使用含有5%甲醇的0.1 M H3BO3（pH 9.5）電解液、18 kV 電壓以及214 nm 偵測波長；MECC的最適條件則為含有0.05M SDS的0.03 M Na2B4O7（pH 8.5）電解液及-15 kV 電壓，同樣採用25℃及214 nm 偵測波長。我們從原膠中發現12 種類黃酮，總含量在254 到19147ppm，但是在蜂膠商品中只測到11 種類黃酮，總含量在1228 到7985 ppm ，這些含量上的差異可能由於不同來源及加工過程所造成。

Flavonoid contents of nine raw and nine commercial propolis samples were analyzed by capillary electrophoresis. At first, 15 commercially available flavonoid standards including apigenin, chrysin, luteolin, galangin, kaempferol, morin, myricetin, quercetin, quercitrin, rutin, hesperetin, naringin, naringenin, daidzein and genistein were used to develop the electrophoretic conditions. At a running temperature of 25°C and detection wavelength at 214 nm, the separation of these standards was most satisfactorily achieved by a combination of capillary zone electrophoresis (CZE), utilizing 0.1 M borate buffer (pH 9.5) containing 5% methanol under a voltage of 18 kV, and micellar electrokinetic capillary electrophoresis (MECC), utilizing 0.03 M sodium borate (pH 8.5) containing 0.05 M SDS under a voltage of -15 kV. We found 12 out of the 15 flavonoids were identified in raw propolis, but only 11 were detected in commercial propolis products. The total contents of the identified flavonoids in raw and commercial propolis were 254 to 19147 ppm and 1228 to 7985 ppm, respectively, suggesting that both the origin and processing are important factors affecting the flavonoid content in propolis.