以核醣體去氧核糖核酸之核苷酸序列分析三個石斛品種   全文下載

The rDNA Sequence Analysis of Three Dendrobium Species

正確而精準的分子鑑定基源是避免藥用植物誤用的必要步驟。石斛藥材自古即為著名的治眼疾中藥材，然而石斛藥材的種類繁多，品種紊亂，再加上加工處理，導致外形辨認不易。本研究收集三個石斛品種：銅皮、黃花及金釵石斛，分析部分核醣體去氧核糖核酸核?酸序列，建立rDNA序列資料庫，並分析其歧異度，瞭解其親緣關係，作為今後鑑別的參考。以聚合?連鎖反應擴增部分核醣體去氧核糖核酸區域之ITS 1、5.8S及ITS 2產物，銅皮石斛的長度為632個核?酸，黃花石斛的長度為627個核?酸，而金釵石斛的長度為628個核?酸，其品種間之序列相似度從91%至95%不等。該結果將有助於石斛品種的鑑定。

Positive molecular identification of medicinal plants is necessary to their misuse. Dendrobii Caulis is a famous Chinese herbal medicine traditionally used in ophthalmology. However, the identification of the species based on morphology is difficult. In this study, three Dendrobium species were collected and differentiated by the sequences of partial rDNA (ribosomal DNA) sections which contain the ITS 1, 5.8S, and ITS 2 regions. The Dendrobium species used in this reseach included Dendrobium tosaense, Dendrobium officinale, and Dendrobium moniliforme. The length of PCR products are 632 nt for D. tosaense, 627 nt for D. officinale, and 628 nt for D. moniliform, respectively. The similarities of the rDNA region between the species pairs shown range from 91% to 95%. The results we present here may be helpful in identifying other species of Dendrobium.