中草藥萃取物對白血病U937細胞之抑制及分化作用   全文下載

Growth Inhibition and Differential Induction of Human Leukemic U937 Cells by Chinese Medicinal Herbs

本實驗探討熱水及80%乙醇萃取之中草藥對U937細胞之抑制及分化作用。篩選模式為以多種不同濃度（200-800μg／mL）的中草藥刺激人類周邊血液單核細胞，再取其單核細胞條件培養液（mononuclear cell-condition medium. MNC-CM）刺激U937細胞。結果顯示，黃耆、甘草、蓮子、棗、黨參熱水萃取物之單核細胞條件培養液（MNC-CM）在高劑量時可有效抑制U937細胞之生長（>40%），但其乙醇萃取物之單核細胞條件培養液效果則較熱水萃取物差。而不論熱水或乙醇萃取物均無直接抑制U937細胞分化之作用。在NBT還原試驗中，蓮子熱水萃取物經酒精沉澱後所得之酒精沉澱物（100-400μg／mL）之還原力隨著添加濃度的增加而有增加的趨勢（31-49%），顯示蓮子熱水萃取之酒精沉澱物的條件培養液有明顯誘導、分化U937細胞成熟的能力。

Effects of boiling-water extracts and 80% (v/v) ethanol extracts from Chinese medicinal herbs on the antiproliferation and differentiation of human leukemic U937 cells were examined. Conditioned medium from peripheral blood monocytes (MNC-CM) stimulated with various levels (200-800 μg/mL) and types of herb extracts (MNC-CM mode) were used to screen the effective herbs. Results showed that among the herbs tested, MNC-CM from boiling-water extracts of Huang Gi (HG), Gan Cao (GC), Lian Zi (LZ), Da Zao (DZ), and Tang Shen (TS) showed remarkable (> 40%) growth inhibition on the U937 cells at high dosage, whereas mixtures of boiling-water extracts of tested herbs with culturing medium displayed insignificant results. Meanwhile, inferior inhibition on cell proliferation was observed when U937 cells were treated with ethanol extracts with MNC-CM or conventional mode. Assays for cytoplasmic superoxide production (NBT reducing ability) by U937 cells induced by ethanol precipitates of boiling-water extracts (100-400 μg/mL) from LZ indicated a dose-dependent positive percentage (31-49%), revealing the apparent differentiation-inducing effect of MNC-CM on U937 cells.