氟硫滅在芥藍菜與包心白菜上之殘留量分析   全文下載

Flusulfamide Residue Detection in Chinese Kale and Cabbage

氟硫滅屬於sulfonanilide類之殺菌劑，其在台灣被應用於防治由Plamodiphora brassicae Woronin所引起的色心白菜根瘤病。目前有關氟硫滅在作物上殘留量分析方法之文獻報告甚少發表，因此本研究的目的在於建立氟硫滅於色心白菜及芥藍菜上之分析方法，以提供日後研究此類藥劑殘留量時之參考。先以丙酮萃取後，接著以液-液萃取將分析物轉入二氯甲烷中，經矽酸固相萃取淨化後， 再以碘化鉀烷進行氟硫滅之甲基化反應， 經矽酸鎂固相萃取淨化後，以氣相層析儀附電子捕獲式檢出器偵測之，並以氣相層析質譜儀確認其結構，氟硫滅於芥藍菜及包心白菜上之回收率分別為92.6%及93.2%，偵測界限皆為0.001μg／mL。

Flusulfamide, a sulfonanilide family fungicide, is used to control clubroot caused by Plamodiophora brassicae Woronin in Chinese cabbage in Taiwan. To know the amounts of residues on the crops are very important in our IES (Inspection and Education System); unfortunately, information on analytical methods to determine flusulfamide residues is very rare. An analytical method was developed to detect the flusulfamide residues in Chinese kale and Chinese cabbage. The residue of sulfonanilide in vegetable samples were extracted by acetone and partitioned to dichloromethane and cleaned up by SPE (solid phase extraction). The residue was further methylated with methyl iodide. The derivatives were measured by GC-ECD and identified by GC/MS. The recovery of flusulfamide in Chinese kale and Chinese cabbage were 92.6 and 93.2% by fortification with 0.005 to 0.5 μg/g, respectively. The detection limits were 0.001 μg/g for crops. The method was applied to the determination of flusulfamide residues in crops from treated field. 氟硫滅屬於sulfonanilide類之殺菌劑，其在台灣被應用於防治由Plamodiphora brassicae Woronin所引起的色心白菜根瘤病。目前有關氟硫滅在作物上殘留量分析方法之文獻報告甚少發表，因此本研究的目的在於建立氟硫滅於色心白菜及芥藍菜上之分析方法，以提供日後研究此類藥劑殘留量時之參考。先以丙酮萃取後，接著以液-液萃取將分析物轉入二氯甲烷中，經矽酸固相萃取淨化後， 再以碘化鉀烷進行氟硫滅之甲基化反應， 經矽酸鎂固相萃取淨化後，以氣相層析儀附電子捕獲式檢出器偵測之，並以氣相層析質譜儀確認其結構，氟硫滅於芥藍菜及包心白菜上之回收率分別為92.6%及93.2%，偵測界限皆為0.001μg／mL。