| 中文摘要 |
使用高效液相層析（HPLC）進行鼻良湯之成分分析，開發出包含川芎中ferulic acid、芍藥中paeoniflorin、甘草中glycyrrhizin、肉桂中cinnamic acid, cinnarnaldehyde、葛根中puerarin, daidzin及黃芩中baicalin, baicalein等九種指標成分同時定量分析方法。鼻良湯之試料通過保持在30℃恆溫之HPLC層析管（Inertsil ODS-2, 4.6 mm i.d. × 250 mm），移動相採用20%，50%及90% acetonitrile之混和溶液，並用磷酸調整pH值為3.0，進行梯度沖提法，以1.0 mL／min之流速沖提。九種指標成分之檢測使用UV偵測器，偵測波長設定230 nm。本法之回收率，同日間及異日間的變異係數均在5%以下，相對誤差各低於2.63%及1.81%，回收率在90-108.67%之間。這個分析法對於鼻良湯製劑中九種指標成分是安定且值得信賴之定量法。" |
| 英文摘要 |
Simultaneous determination of nine marker substances was established for the quality control of “Byi-Liang-Tang” by high performance liquid chromatography (HPLC). These marker substances include ferulic acid from Cnidii Rhizoma, paeoniflorin from Paeoniae Radix, glycyrrhizin from Glycyrrhizae Radix, cinnamic acid and cinnamaldehyde from Cinnamomi Cortex, puerarin and daidzin from Puerariae Radix, and baicalin and baicalein from Scutellariae Radix. Extracted samples were analyzed with reverse-phase column (Inertsil 5 ODS-2, 4.6 mm i.d. × 250 mm) at 30˚C and eluted with a mixture of 20%, 50% and 90% acetonitrile (adjusted to pH 3.0 with phosphoric acid) aqueous solution in gradients at a flow-rate of 1.0 mL/min, and detected at 230 nm. Relative coefficients of variations of intra- and inter-day analysis were less than 5% and the relative errors were below 2.63% and 1.81%, respectively. All the recoveries were 90~108.67%. This method could be applied for the simultaneous determination of nine marker substances in “Byi-Liang-Tang” and comparisons to commercially preparations.
使用高效液相層析（HPLC）進行鼻良湯之成分分析，開發出包含川芎中ferulic acid、芍藥中paeoniflorin、甘草中glycyrrhizin、肉桂中cinnamic acid, cinnarnaldehyde、葛根中puerarin, daidzin及黃芩中baicalin, baicalein等九種指標成分同時定量分析方法。鼻良湯之試料通過保持在30℃恆溫之HPLC層析管（Inertsil ODS-2, 4.6 mm i.d. × 250 mm），移動相採用20%，50%及90% acetonitrile之混和溶液，並用磷酸調整pH值為3.0，進行梯度沖提法，以1.0 mL／min之流速沖提。九種指標成分之檢測使用UV偵測器，偵測波長設定230 nm。本法之回收率，同日間及異日間的變異係數均在5%以下，相對誤差各低於2.63%及1.81%，回收率在90-108.67%之間。這個分析法對於鼻良湯製劑中九種指標成分是安定且值得信賴之定量法。 |
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