利用高效液相層析法定量美白化粧品中Magnesium ascorbyl phosphate、Ascorbyl glucoside、Kojic acid、Arbutin及Hydroquinone等成分   全文下載

Simultaneous Determination of Magnesium Ascorbyl Phosphate, Ascorbyl Glucoside, Kojic Acid, Arbutin and Hydroquinone in Skin Whitening Cosmetics by HPLC

本研究係利用高效液相層析法，同時分析衛生署公告得添加於化粧品中之四種美白成分：Magnesiurn ascorbyl phosphate、Ascorbyl glucoside、Kojic acid、Arbutin及不得添加之Hydroquinone（對苯二酚）等五種成分。檢體先經0.05 M KH2PO4溶液（pH 2.5）抽取，以高效液相層析儀進行分析。使用之層析管柱為COSMOSIL 5C18-AR-II （4.6 mm i.d. × 250 mm），移動相為0.05 M KH2PO4 （pH2.5） 甲醇（99：1，v／v），檢測波長為280 nm，並以pyridoxine為內部標準品。五種成分標準品所得五條標準曲線之線性回歸顯示相關係數，R2介於0.9998-1.0000之間，而同日內及異日間試驗之相對標準偏差皆小於2.4%。添加不同濃度之標準品於空白檢體中，其回收率介於93.5~103.3%，相對標準偏差皆小於1.3%。Magnesium ascorbyl phosphate、Ascorbyl glucoside、Kojic acid、Arbutin及Hydroquinone之最低定量濃度則分別為80.0、20.0、3.0、15.0及10.0μg／mL。

A high performance liquid chromatographic method was developed for simultaneous determination of 5 whitening ingredients: magnesium ascorbyl phosphate, ascorbyl glucoside, kojic acid, arbutin and hydroquinone decomposed from arbutin in cosmetics. Samples were extracted with 0.05 M KH2PO4 buffer solution (pH 2.5) and analyzed using a Cosmosil 5 C18-AR-II column. A mixture of 0.05 M KH2PO4 buffer solution (pH 2.5) and methanol (99:1, v/v) was used as mobile phase. The UV detector was set at 280 nm. Pyridoxine was used as an internal standard. The related coefficients, R2, of regression equations of the 5 standard curves were 0.9998- 1.0000. The relative standard deviations of the 5 ingredients for intraday and interday analysis were less than 2.4%. The average recoveries of these 5 ingredients spiked in sample ranged from 93.5% to 103.3%. The relative standard deviations of average recoveries were less than 1.3%. The limits of quantitation in cosmetics were 80.0, 20.0, 3.0, 15.0 and 10.0 μg/mL for magnesium ascorbyl phosphate, ascorbyl glucoside, kojic acid, arbutin and hydroquinone, respectively. 本研究係利用高效液相層析法，同時分析衛生署公告得添加於化粧品中之四種美白成分：Magnesiurn ascorbyl phosphate、Ascorbyl glucoside、Kojic acid、Arbutin及不得添加之Hydroquinone（對苯二酚）等五種成分。檢體先經0.05 M KH2PO4溶液（pH 2.5）抽取，以高效液相層析儀進行分析。使用之層析管柱為COSMOSIL 5C18-AR-II （4.6 mm i.d. × 250 mm），移動相為0.05 M KH2PO4 （pH2.5） 甲醇（99 : 1，v／v），檢測波長為280 nm，並以pyridoxine為內部標準品。五種成分標準品所得五條標準曲線之線性回歸顯示相關係數，R2介於0.9998-1.0000之間，而同日內及異日間試驗之相對標準偏差皆小於2.4%。添加不同濃度之標準品於空白檢體中，其回收率介於93.5～103.3%，相對標準偏差皆小於1.3%。Magnesium ascorbyl phosphate、Ascorbyl glucoside、Kojic acid、Arbutin及Hydroquinone之最低定量濃度則分別為80.0、20.0、3.0、15.0及10.0μg／mL。