台灣西部產大玉螺與細紋玉螺之毒性學研究   全文下載

Occurrence of Tetrodotoxin in the Causative Gastropod Polinices didyma and another Gastropod Natica lineata Collected from Western Taiwan

在2000年7月，嘉義地區發生因食用大玉螺而導致之食物中毒案件。為確實了解嘉義產食用螺類之毒成分、毒量和可能導致之危險性，乃採集經煮熟之中毒大玉螺檢體並每月收集新鮮之大玉螺及細紋玉螺進行河魨毒檢測分析。樣品經河魨毒生物檢定法檢測毒量，並將貝類之醋酸-甲醇抽出物經超過濾及Bio-Gel P-2膠體過濾純化後，進行高效能液相層析儀和氣相層析儀之定性分析。結果得知大玉螺與細紋玉螺均含河魨毒，且煮熟之大玉螺檢體與新鮮樣品之平均個體毒量分別為117.8 ± 105.3與47.0 ± 28.3 MU。因此確認造成此食物中毒事件之原因物質為河魨毒。

Attempts were made to elucidate the responsible toxins in the gastropod Polinices didyma which recently caused food poisoning incidents in western Taiwan. The remained (cooked) and captured (live) specimens of both gastropods were assayed for toxicity (as tetrodotoxin = TTX). Average toxicity of cooked and live specimens was 118 ± 105 and 47 ± 28 MU/specimen, respectively. In addition, another species Natica lineata collected from the same area was also found to be toxic. The toxin of each gastropod was partially purified from the methanolic extract of the gastropod by ultrafiltration and Bio-Gel P-2 column chromatography. HPLC and GC-MS analyses demonstrated that the toxin consisted of TTX. It was concluded that the causative agent of the above food poisoning was TTX. 在2000年7月，嘉義地區發生因食用大玉螺而導致之食物中毒案件。為確實了解嘉義產食用螺類之毒成分、毒量和可能導致之危險性，乃採集經煮熟之中毒大玉螺檢體並每月收集新鮮之大玉螺及細紋玉螺進行河魨毒檢測分析。樣品經河魨毒生物檢定法檢測毒量，並將貝類之醋酸-甲醇抽出物經超過濾及Bio-Gel P-2膠體過濾純化後，進行高效能液相層析儀和氣相層析儀之定性分析。結果得知大玉螺與細紋玉螺均含河魨毒，且煮熟之大玉螺檢體與新鮮樣品之平均個體毒量分別為117.8 ± 105.3與47.0 ± 28.3 MU。因此確認造成此食物中毒事件之原因物質為河魨毒。