腸炎弧菌偵測與分子生物分型方法   全文下載

Detecting and Molecular Typing of Vibrio parahaemolyticus

腸炎弧菌是亞洲國家非常流行的食品中毒菌。本又回顧腸炎弧菌的傳統與新發展的培養、鑑定、計數和快速偵測病原因子的方法，所偵測的致病因子主要為耐熱溶血素和它相關之溶血素和不耐熱溶血素。本文同時回顧為了流行病學分析所使用的各類分子分型方法，如脈衝式電泳、核糖體基因多形性、隨機引子增幅和其他多種核酸聚合脢反應分型方法等，這些方法針對專一性與保守的區域設計引子。

Vibrio parahaemolyticus is a prevalent food-borne pathogen in many Asian countries and has been spreading rapidly following the appearance of pandemic strains. This article reviews conventional and molecular methods for culturing, identifying, enumerating and rapidly detecting V. parahaemolyticus and its virulence factors, such as thermostable direct hemolysin (TDH), TDH-related hemolysin and thermolabile hemolysin. It also discusses various molecular typing methods, namely, pulsed-field gel electrophoresis, ribotyping, random amplified polymorphic DNA and three other polymerase chain reaction methods based on specific conserved nucleotide sequences, such as ribosomal gene spacer, repetitive extragenic palindromic and enterobacterial repetitive intergenic consensus sequences, for use in epidemiological investigations of this pathogen. 腸炎弧菌是亞洲國家非常流行的食品中毒菌。本又回顧腸炎弧菌的傳統與新發展的培養、鑑定、計數和快速偵測病原因子的方法，所偵測的致病因子主要為耐熱溶血素和它相關之溶血素和不耐熱溶血素。本文同時回顧為了流行病學分析所使用的各類分子分型方法，如脈衝式電泳、核糖體基因多形性、隨機引子增幅和其他多種核酸聚合脢反應分型方法等，這些方法針對專一性與保守的區域設計引子。