化粧品中α-Hydroxyacids之分析   全文下載

Determination of α-Hydroxyacids in Cosmetics

本研究利用高效液相層析法，分析化粧品中四種α-Hydroxyacids（AHAs）（Glycolic acid、dl-Malic acid、Lactic acid、Citric acid）之含量，本實驗採用C18逆相層析管柱Capcell PAK C18 UGl20 S-5μm （4.6 × 250 mm），移動相為2%磷酸溶液（pH 2.0），內部標準品為Maleic acid，檢測波長為210 mn，得到良好分析結果。四種AHAs於50-500μg／mL均呈現良好線性關係，其相關係數（r）為介於0.9992~0.9995之間，四種AHAs之同日內及異日間試驗之相對標準偏差，分別為0.05~1.49%及0.72~3.24%，四種AHAs之添加回收率為介於96.3%至99.2%之間。

A high performance liquid chromatographic method was developed for the simultaneous determination of four α-hydroxyacids (AHAs, Glycolic acid, dl-Malic acid, Lactic acid and Citric acid) in cosmetics. Samples were analyzed using a reverse-phase C18 column (Capcell PAK C18 UG120 S-5 μm) with 2% phosphoric acid (pH 2.0) as mobile phase at 210 nm. Calibration curves of four α-hydroxyacids were constructed in the range of 50-500 μg/mL and their correlation coefficients were in the range of 0.9992-0.9995. The relative standard deviations of four α-hydroxyacids for intraday and interday analyses were 0.05~1.49% and 0.72~3.24%, respectively. The average recoveries of four α-hydroxyacids ranged from 96.3% to 99.2%. 本研究利用高效液相層析法，分析化粧品中四種α-Hydroxyacids（AHAs）（Glycolic acid、dl-Malic acid、Lactic acid、Citric acid）之含量，本實驗採用C18逆相層析管柱Capcell PAK C18 UGl20 S-5μm （4.6 × 250 mm），移動相為2%磷酸溶液（pH 2.0），內部標準品為Maleic acid，檢測波長為210 mn，得到良好分析結果。四種AHAs於50-500μg／mL均呈現良好線性關係，其相關係數（r）為介於0.9992～0.9995之間，四種AHAs之同日內及異日間試驗之相對標準偏差，分別為0.05～1.49%及0.72～3.24%，四種AHAs之添加回收率為介於96.3%至99.2%之間。