以高效液相層析儀檢測馬鈴薯中抑芽素殘留量   全文下載

High Performance Liquid Chromatographic Determination of Maleic Hydrazide Residue in Potatoes

以甲醇萃取馬鈴薯中抑芽素之殘留量，經過濾或離心，抽出液以減壓濃縮至乾，殘留物以水溶解後，以SCX固相萃取匣（a strong cation exchanger）淨化之，所得檢液以高效液相層析儀配合螢光檢出器偵測。抑芽素添加10~20 ppm 檢體濃度於馬鈴薯中之平均回收率為87.8~95.7%，變異係數為2.1~4.0%。本方法操作簡便、回收率高且再現性良好，最低檢出限量為0.5 ppm 。

A reliable and fast method was developed using high performance liquid chromatography equipped with a fluorescence detector for separating and quantifying the residues of maleic hydrazide in potatoes. The method started with methanol extraction, followed by filtration or centrifugation, and concentration to dryness. The remaining was reconstituted with water for cleanup, which was applied to a SCX solid phase extraction cartridge (a strong cation exchanger). Average recoveries of potatoes spiked with 10~20 ppm maleic hydrazide were in the range of 87.8~95.7%, and their coefficients of variation were in the range of 2.1~4.0%. The limit of detection was 0.5 ppm. 以甲醇萃取馬鈴薯中抑芽素之殘留量，經過濾或離心，抽出液以減壓濃縮至乾，殘留物以水溶解後，以SCX固相萃取匣（a strong cation exchanger）淨化之，所得檢液以高效液相層析儀配合螢光檢出器偵測。抑芽素添加10～20 ppm 檢體濃度於馬鈴薯中之平均回收率為87.8～95.7%，變異係數為2.1～4.0%。本方法操作簡便、回收率高且再現性良好，最低檢出限量為0.5 ppm 。