麻黃生物鹼之逆相層析探討   全文下載

Separation of the Ephedra Alkaloids by RPLC

搭配ODS管柱的逆相高效液相層析（RPLC）是目前最常用來分析中藥材指標成分的技術。本文旨在以三不同ODS材質的逆相層析管柱，探討不同SDS（sodium dodecyl sulfate）濃度與品牌、有機溶劑含量及酸鹼值對麻黃指標成分麻黃素（（—）-ephedrine）與偽麻黃素（（+）-pseudoephedrine）之滯留時間的影響。

High-performance liquid chromatography with ODS columns has been the most popular technique in analyzing the Chinese herbal drugs. (–)-Ephedrine and (+)-pseudo-ephedrine are the major index components in Ephedrae Herba. In order to separate these two alkaloids, three ODS columns were evaluated. The relationships between SDS concentrations, brands, organic solvent ratios and pH values and retention times of the ephedrine and pseudoephedrine were also studied. 搭配ODS管柱的逆相高效液相層析（RPLC）是目前最常用來分析中藥材指標成分的技術。本文旨在以三不同ODS材質的逆相層析管柱，探討不同SDS（sodium dodecyl sulfate）濃度與品牌、有機溶劑含量及酸鹼值對麻黃指標成分麻黃素（（—）-ephedrine）與偽麻黃素（（+）-pseudoephedrine）之滯留時間的影響。