紫雲膏製劑中多成分同時分析研究   全文下載

Simultaneous Determination of Five Constituents in “Tzyy-Yun-Gau” Medicine by High Performance Liquid Chromatography

使用HPLC進行傳統中藥軟膏製劑紫雲膏之成分分析，開發出包含紫草中shikonin, deoxyshikonin, β,β-dimethylacrylshikonin, acetylshikonin及當歸中ferulic acid等五種指標成分之多成分同時定量分析方法。紫雲膏經採用n-hexane 及methanol 二種溶媒，進行分配並取methanol 層為HPLC分析之對象。將製備之試料通過保持在30 ℃恒溫之HPLC層析管（Inertsil ODS-2, 4.6 mm I.D. × 250 mm），移動相採用methanol, acetonitrile 及2% acetic acid 之混合溶液，進行梯度沖提法，以1.0 mL ／分之流速沖提。五種指標成分之檢測使用UV檢出器，檢測波長設定0 ? 25 分為325 nm； 25 ? 58.5 分為525 nm； 58.5? 62 分為440 nm；最後62 ? 80分為525 nm。這個分離法對於紫雲膏中五種指標成分是精確且值得使用之定量法。"

An HPLC method was applied for the simultaneous determination of five marker substances in the traditional Chinese medicine preparation “Tzyy-Yun-Gau”. These substances included shikonin, deoxyshikonin, β,β-dimethylacrylshikonin, and acetylshikonin in Arnebiae Radix; and ferulic acid in Angelicae Radix. Tzyy-Yun-Gau was partitioned in a mixture of n-hexane and methanol, and a fraction taken into the methanol layer was analyzed. The samples were run through an HPLC column (Inertsil ODS-2, 4.6 mm I.D. × 250 mm) at 30°C with a mobile phase consisting of methanol, acetonitrile and 2% acetic acid with linear gradient elution at a flow-rate of 1.0 mL/min. A UV detector was used for detection. The detection wavelength varied with time, which was 325 nm during 0~25 min, 525 nm during 25~58.5 min, 440 nm during 58.5~62 min, and 525 nm during 62~80 min. This could be a successful separation method for the simultaneous determination of five marker substances in “Tzyy-Yun-Gau”. 使用HPLC進行傳統中藥軟膏製劑紫雲膏之成分分析，開發出包含紫草中shikonin, deoxyshikonin, β,β-dimethylacrylshikonin, acetylshikonin及當歸中ferulic acid等五種指標成分之多成分同時定量分析方法。紫雲膏經採用n-hexane 及methanol 二種溶媒，進行分配並取methanol 層為HPLC分析之對象。將製備之試料通過保持在30 ℃恒溫之HPLC層析管（Inertsil ODS-2, 4.6 mm I.D. × 250 mm），移動相採用methanol, acetonitrile 及2% acetic acid 之混合溶液，進行梯度沖提法，以1.0 mL ／分之流速沖提。五種指標成分之檢測使用UV檢出器，檢測波長設定0 ∼ 25 分為325 nm； 25 ∼ 58.5 分為525 nm； 58.5∼ 62 分為440 nm；最後62 ∼ 80分為525 nm。這個分離法對於紫雲膏中五種指標成分是精確且值得使用之定量法。