以石墨爐式原子吸收光譜法測定食用油脂類中銅之含量   全文下載

Determination of Copper in Edible Oils by Direct Graphite Furnace Atomic Absorption Spectrometry

本研究探討以石墨爐式原子吸收光譜儀直接分析食用油脂類中之銅含量，將檢體以2%lecithin-cyclohexane溶液等量稀釋後，以偏極化茲曼石墨爐式原子吸收光譜儀直接檢測，得知最適灰化溫度為900°C，最適原子化溫度為2700°C。銅標準溶液之稀釋液為2% lecithin-cyclohexane優於沙拉油和cyclohexane。Lecithin在cyclohexane之濃度高於2%時，吸光度檢測狀況最佳。本方法之油脂樣品偵測極限為2.0 ng／mL，其次，添加銅10，20及40 ng／mL於大豆沙拉油中，使用標準添加法測定，其回收率為85.5%～90.0%。添加於豬油中，回收率為86.0%～93.0%。

A simple method for determination of copper in edible oils was developed, using a polarized Zeeman graphite furnace atomic absorption spectrophotometer and a pyro graphite tube. Edible oils were diluted with 2%lecithin-cyclohexane and analyzed directly by the standard addition method. The optimal ashing and atomizing temperatures were 900°C and 2700°C, respectively. A standard solution diluted with 2% lecithin-cyclohexane was superior to salad oil or cyclohexane. Results were best measured when the concentration of lecithin in cyclohexane was higher than 2%. The detection limit of this method was 2.0 ng/mL. The respective range of recovery of copper was 85.5~90.0% and 86.0 ~ 93.0% in salad oil and lard which were fortified with 10, 20 and 40 ng Cu/mL respectively using the standard addition method. 本研究探討以石墨爐式原子吸收光譜儀直接分析食用油脂類中之銅含量，將檢體以2%lecithin-cyclohexane溶液等量稀釋後，以偏極化茲曼石墨爐式原子吸收光譜儀直接檢測，得知最適灰化溫度為900°C，最適原子化溫度為2700°C。銅標準溶液之稀釋液為2% lecithin-cyclohexane優於沙拉油和cyclohexane。Lecithin在cyclohexane之濃度高於2%時，吸光度檢測狀況最佳。本方法之油脂樣品偵測極限為2.0 ng／mL，其次，添加銅10，20及40 ng／mL於大豆沙拉油中，使用標準添加法測定，其回收率為85.5%～90.0%。添加於豬油中，回收率為86.0%～93.0%。