防風Cimifugin之製備與HPLC品質管制   全文下載

Cimifugin Preparation and Quantitative Analysis of Saposhnikoviae Radix by HPLC

本研究由防風Saposhnikovia divaricata（Turcz.） Schischk.中抽取純化特有之鎮痛、降血壓之活性成分cimifugin作為指標成分，並建立防風藥材中cimifugin的高效液相層析定量分析模式；其分析條件以CH3CN-H2O （18:82）為移動相，流速為1.0 mL／min，以LiChrospher 100 RP-18e （4 × 250 mm）為分析管柱，以波長220 nm檢測，在40°C下進行分析。防風中cimifugin的最佳抽取條件為以20倍量70%甲醇水溶液經超音波震盪40分鐘可得最高產率。利用上述高效率液相層析法定量市售防風藥材中cimifugin的含量，結果cimifugin含量在2.66 × 10-2% 至0.19 × 10-2%之間。 Cimifugin, one of the principal analgesic and anti-hypertensive component of Fang-Feng, was isolated from the root of Saposhnikovia divaricata. Reversed-phase high-performance liquid chromatography was employed to determine the contents of cimifugin in Fang-Feng. The separation was performed on a LiChrospher 100 RP-18e column (4 × 250 mm) by isocratic elution with acetonitrile-water (18:82) as the mobile phase at a flow-rate of 1.0 mL/min, the temperature being kept at 40°C and the detection set at 220 nm. Methylparaben was used as the internal standard. The regression equation revealed linear relationship between the peakarea ratio (marker substance/internal standard) and concentration. We found that Fang-Feng extracted with twenty-fold of 70% aqueous methanol under sonication for 40 minutes gives the best yield of cimifugin. The contents of cimifugin in commercially available Saposhnikoviae Radices have been determined and the amount was found to vary from 2.66 × 10-2% to 0.19 × 10-2%. This HPLC method can rapidly and easily quantify the cimifugin present in Fang-Feng and can be used for quality control of this commercial crude drug."

Cimifugin, one of the principal analgesic and anti-hypertensive component of Fang-Feng, was isolated from the root of Saposhnikovia divaricata. Reversed-phase high-performance liquid chromatography was employed to determine the contents of cimifugin in Fang-Feng. The separation was performed on a LiChrospher 100 RP-18e column (4 × 250 mm) by isocratic elution with acetonitrile-water (18:82) as the mobile phase at a flow-rate of 1.0 mL/min, the temperature being kept at 40°C and the detection set at 220 nm. Methylparaben was used as the internal standard. The regression equation revealed linear relationship between the peakarea ratio (marker substance/internal standard) and concentration. We found that Fang-Feng extracted with twenty-fold of 70% aqueous methanol under sonication for 40 minutes gives the best yield of cimifugin. The contents of cimifugin in commercially available Saposhnikoviae Radices have been determined and the amount was found to vary from 2.66 × 10-2% to 0.19 × 10-2%. This HPLC method can rapidly and easily quantify the cimifugin present in Fang-Feng and can be used for quality control of this commercial crude drug.