紙製容器（餐盤、紙杯及便當盒）中螢光增白劑檢驗方法之探討   全文下載

Studies on Analytical Method for Determination of Fluorescent Whitening Agents in Paper Containers for Food

本研究利用高效液相層析法（high performance liquid chromatography，HPLC）分析紙製容器（餐盤、紙杯及便當盒）中之螢光增白劑（fluorescent whitening agents）。經依據衛生署公告第762034號之螢光增白劑檢驗方法，將紗布浸泡後，以二甲醯胺萃取，經減壓濃縮至乾，轉溶後再以HPLC分離鑑定。發現，螢光增白劑可以下列之HPLC分析條件加以分離鑑定，即以Prodigy 5μm ODS （2）為分離管，乙?：0.01 M磷酸二氫鈉（35:65，v／v）為移動相，以螢光檢測器於激發波長340 nm及放射波長430 nm檢測。其次，以上述條件，分析五十件市售紙製容器，經確認結果均未檢出螢光增白劑。

The method of determination of fluorescent whitening agents (FWA) in paper containers for food was studied. Samples were assessed in accordance with official procedures. The treated gauze was extracted by dimethylformamide, evaporated to dryness, and then analyzed by HPLC. The extracted FWAs were separated with Prodigy 5µm ODS (2) column using acetonitrile: 0.01 M sodium dihydrogen phosphate (35:65) as solvent. For spectrofluorimetric detection, an excitation wavelength of 340 nm and emission wavelength of 430 nm were used. Applying the above HPLC method, fifty samples of paper containers for food were analyzed. No residue of FWA was detected in any sample. 本研究利用高效液相層析法（high performance liquid chromatography，HPLC）分析紙製容器（餐盤、紙杯及便當盒）中之螢光增白劑（fluorescent whitening agents）。經依據衛生署公告第762034號之螢光增白劑檢驗方法，將紗布浸泡後，以二甲醯胺萃取，經減壓濃縮至乾，轉溶後再以HPLC分離鑑定。發現，螢光增白劑可以下列之HPLC分析條件加以分離鑑定，即以Prodigy 5μm ODS （2）為分離管，乙腈：0.01 M磷酸二氫鈉（35:65，v／v）為移動相，以螢光檢測器於激發波長340 nm及放射波長430 nm檢測。其次，以上述條件，分析五十件市售紙製容器，經確認結果均未檢出螢光增白劑。