以氣相層析質譜儀分析尿中大麻代謝物   全文下載

Gas Chromatography-Mass Spectrometry Analysis of 11-Nor-9-carboxy-Δ9-Tetrahydrocannabinol in Urine

本研究係以氣相層析質譜分析法來定量尿中大麻代謝物11-nor-9-carboxy-Δ9-tetrahydro-cannabinol （THC-COOH）。經評估d3-與d9-THC-COOH二種內標準品後，發現d9-THC-COOH對尿中大麻代謝物並無任何干擾定量分析之離子，適合GC／MS做定量分析。本實驗採三甲基矽化（trimethylsilyl）衍生物，以離子監測法（SIM, SelectedIonMonitoring）來定量，監測離子THC-COOH為m／z 371, 473, 488，內標準d9-THC-COOH為m／z 380, 479, 497。以2ml空白尿液配製濃度在10至200 ng／ml範圍內，其抽取回收率平均約為90%，日間與日內精密度試驗，其變異係數分別為0.35～5.31%與0.44～5.19%，並具有良好的線性關係r2>0.999。"

Effective specimen pretreatment and GC-MS protocols were developed for the analysis of 11-nor-9-carboxy-Δ9 -tetrahydrocannabinol (THC-COOH) in urine. d3- and d9-11-nor-9-carboxy-Δ9-tetrahydrocannabinol (d3- and d9-THC-COOH) were evaluated as internal standards and conclusions were reached that THC-COOH can be effectively analyzed, qualitatively and quantitatively, as trimethylsilyl derivative using selective ion monitoring of the following ions: m/z 371, 473 and 488 (for THC-COOH) and m/z 380, 479 and 497 (for d9-THC-COOH). The extraction procedure achieved 90o/o recovery of the analyte in fortified drug-free urine containing 10-200 ng/ml THC-COOH. Using 2-ml sample size, the overall protocol resulted in the following analytical parameters in the 10-200 ng/ml concentration range studied: interday and intraday precision ranges, 0.35-5.31% and 0.44-5.19%, respectively; linearity, r2 > 0.999. 本研究係以氣相層析質譜分析法來定量尿中大麻代謝物11-nor-9-carboxy-Δ9-tetrahydro-cannabinol （THC-COOH）。經評估d3-與d9-THC-COOH二種內標準品後，發現d9-THC-COOH對尿中大麻代謝物並無任何干擾定量分析之離子，適合GC／MS做定量分析。本實驗採三甲基矽化（trimethylsilyl）衍生物，以離子監測法（SIM, SelectedIonMonitoring）來定量，監測離子THC-COOH為m／z 371, 473, 488，內標準d9-THC-COOH為m／z 380, 479, 497。以2ml空白尿液配製濃度在10至200 ng／ml範圍內，其抽取回收率平均約為90%，日間與日內精密度試驗，其變異係數分別為0.35～5.31%與0.44～5.19%，並具有良好的線性關係r2>0.999。