高效液相層析探討黑米之花青素   全文下載

High Performance Liquid Chromatographic Determination of Anthocyanin Pigments in Some Varieties of Black Rice

用纖維薄層層析及製備級的高效液相層析將不同品種的黑米中的花青素分離出來，並以色層分析來鑑定。Cyanidin 3-glucoside和penonidin 3-glucoside是黑米中最重要的兩種花青素。所有品種的花青素總量分析介於0 ~ 493 mg／100 g 的米粒。Cyanidine 3-glucoside是一個重要的自由基收容物（ORAC）。在所有檢驗的黑米品種中以Suwon #415 品種的Cyanidine 3-glucoside含量最高。

Anthocyanin pigments in ten pigmented rice cultivars were isolated with cellulose thinlayer chromatography and preparative high performance liquid chromatography (HPLC), and further identified with conventional chromatographic techniques. Individual anthocyanins were then quantified by HPLC using a reversed ODS-5 column and detected at 280 nm. Ten rice varieties contained two major anthocyanins, cyanidin 3-glucoside and peonidin 3-glucoside. Total anthocyanin contents varied greatly in the range of 0-493 mg/100 g grain among varieties. Cyanidin 3-glucoside, an agent having important oxygen radical absorbing capacity (ORAC), was most abundant in Suwon #415 variety (470 mg/100 g grain), while Jawangdo (10 mg/100 g grain) and Ilpumbyeo (not detected) had the smallest amount of anthocyanin among the samples examined. As for the distribution ratio of individual anthocyanin, Suwon #415 was characterized by a relatively high percentage of cyanidin 3-glucoside (95.3%); whereas, Suwon #425 and Heugjinmi were characterized by a relatively larger amount of peonidin 3-glucoside. 用纖維薄層層析及製備級的高效液相層析將不同品種的黑米中的花青素分離出來，並以色層分析來鑑定。Cyanidin 3-glucoside和penonidin 3-glucoside是黑米中最重要的兩種花青素。所有品種的花青素總量分析介於0～493 mg／100 g 的米粒。Cyanidine 3-glucoside是一個重要的自由基收容物（ORAC）。在所有檢驗的黑米品種中以Suwon #415 品種的Cyanidine 3-glucoside含量最高。