以高效液相層析質譜儀分析紅茶中的茶黃質   全文下載

Rapid Analysis of Theaflavins by High Performance Liquid Chromatography / Atmospheric Pressure Chemical Ionization-Mass Spectrometry

本研究乃以10 cm × 4.6 mm i.d. SilicaROD RP-18 高效液相層析（HPLC）分離管柱，配合乙?-2%醋酸或乙?-1%檸檬酸之流動相系統作等梯度沖提，可有效並快速分離紅茶中的茶黃質。其分析時間可於10分鐘內完成。茶黃質的鑑定工作乃以HPLC配合大氣壓化學離子化-質譜儀（APCI-MS） 進行。利用正離子源作離子化，可於質譜圖上觀察到茶黃質的類分子離子（quasi-molecular ions, MH+）峰及一些特殊的斷裂離子峰。本報告並提出茶黃質質譜斷裂離子峰之可能形成途徑。"

A 10 em × 4.6 mm i.d. SilicaROD RP-18 HPLC column coupled with isocratic elution with a solvent system of acetonitrile-2% acetic acid or acetonitrile-1% citric acid were used to analyze purchased theaflavin mixtures and theaflavin extracts from black tea. Individual theaflavins were well separated and the analysis of theaflavins was achieved in 10 min using this method. HPLC coupled with Atmospheric Pressure Chemical Ionization-Mass Spectrometry (APCI-MS) in the positive ion mode was used to identify individual theaflavins. Quasi-molecular ions and many structural characteristic fragment ions were observed in the mass spectra. Possible fragmentation pathways from the protonated theaflavins were also proposed. 本研究乃以10 cm × 4.6 mm i.d. SilicaROD RP-18 高效液相層析（HPLC）分離管柱，配合乙腈-2%醋酸或乙腈-1%檸檬酸之流動相系統作等梯度沖提，可有效並快速分離紅茶中的茶黃質。其分析時間可於10分鐘內完成。茶黃質的鑑定工作乃以HPLC配合大氣壓化學離子化-質譜儀（APCI-MS） 進行。利用正離子源作離子化，可於質譜圖上觀察到茶黃質的類分子離子（quasi-molecular ions, MH+）峰及一些特殊的斷裂離子峰。本報告並提出茶黃質質譜斷裂離子峰之可能形成途徑。