利用毛細管電泳鑑別燕窩   全文下載

Application of Capillary Electrophoresis for Identification of the Authenticity of Bird's Nests

利用毛細管電泳分析胺基酸組成鑑別燕窩真偽，燕窩檢體經酸及鹼水解後，以dansyl chloride衍生化，再以毛細管電泳分析。胺基酸之回收率（包括NH3在內）為102.30%，檢出限量為0.01～0.03 pmole。另就全省各中藥店及超級市場所購得之十件燕窩藥材及二十件冰糖燕窩製品，進行分析。燕窩藥材之粗蛋白質為47.05～58.91%。冰糖燕窩製品之固形物含量為2.29～54.45%，粗蛋白質為1.44～58.45%。於UV 365 nm下，燕窩藥材多半呈淡藍白色螢光，冰糖燕窩製品較難判定。由胺基酸分析圖譜發現，燕窩真品具相同之胺基酸profile，而冰糖燕窩製品中，一件疑為白木耳及其他物質之混合加工品，五件因未檢出胺基酸可能是以洋菜、燕石或海藻酸鈉製成之替代品，另有一件檢出hydro:xyproline疑係以豬皮加工製成。 A method using capillary electrophoresis (CE) to determine amino acid prfile was developed to identify the authenticity of bird's nest. Bird's nest samples were hydrolyzed in acid and alkali, respectively. The protein hydrolysates were then derivatized with dansyl chloride, and analyzed by CE. The average recovery of amino acids was 102.30%. The detection limits were between 0.01 and 0.03 pmole. Ten bird's nest samples and twenty bird's nest in rock sugar syrup were analyzed. The bird's nest samples were found to contain 47.05~58.91% crude protein, while bird's nest in rock sugar syrup were found to contain 1.44~58.45%. All the bird's nest samples were identified to be authentic based on amino acid profile matching. Among 20 bird's nest in rock sugar syrup, seven were considered to be substituted with agar, natural gum or pork skin. CE analysis of amino acid profile was found to be an efficient and sensitive technique for identifying the authenticity of bird's nests."

A method using capillary electrophoresis (CE) to determine amino acid prfile was developed to identify the authenticity of bird's nest. Bird's nest samples were hydrolyzed in acid and alkali, respectively. The protein hydrolysates were then derivatized with dansyl chloride, and analyzed by CE. The average recovery of amino acids was 102.30%. The detection limits were between 0.01 and 0.03 pmole. Ten bird's nest samples and twenty bird's nest in rock sugar syrup were analyzed. The bird's nest samples were found to contain 47.05~58.91% crude protein, while bird's nest in rock sugar syrup were found to contain 1.44~58.45%. All the bird's nest samples were identified to be authentic based on amino acid profile matching. Among 20 bird's nest in rock sugar syrup, seven were considered to be substituted with agar, natural gum or pork skin. CE analysis of amino acid profile was found to be an efficient and sensitive technique for identifying the authenticity of bird's nests.