市售減脂茶中Sennosides A、B 含量測定   全文下載

Determination of Sennosides A and B in Diet Tea by HPLC

本研究利用高效液相層析儀，分析番瀉葉、番瀉莢果及市售減脂茶中sennoside A及B之含量，採用C 18逆相層析管柱，移動相為乙?～0.1%醋酸溶液，利用線性梯度沖提，檢測波長為270 nm；得到良好分析結果。Sennoside A及B二成分之線性迴歸方程式及相關係數（r）分別為：sennoside A （濃度7.52～37.60μg／ml）, Y=32.07032X-0.1098656 （r=0.9998）；sennoside B （濃度8.32～49.92μg／ml）, Y= 28.13735X + 0.2604257 （r=0.9999），均呈現良好線性關係。添加回收率試驗結果，番瀉葉之sennoside A為100.7 ± 0.90%，sennoside B 為102.3 ± 1.31%；番瀉莢果之sennoside A為106.6 ± 0.50%，sennoside B為102.5 ± 2.17%，顯示準確性佳。同日內及異日間試驗之相對標準偏差，分別為0.24～1.72%及0.23～1.71%，顯示高效液相層析法用於sennoside A及B之分析其結果良好。利用上述高效液相層析法定量番瀉葉及番瀉莢果內sennoside A及B的含量，結果番瀉葉中的sennoside A及B含量分別為0.64、1.57%，番瀉莢果中的sennoside A及B的含量分別為1.46、2.30%。依此法分析不同七種市售減脂茶中sennoside A及B的含量，結果sennoside A 之含量則在0.10～0.19%之間；sennoside B之含量在0.16～0.28%之間。 A rapid high performance liquid chromatographic method was developed for determination of sennosides A and B in senna leaves, pods and diet tea. The determination was carried out by using a gradient elution with acetonitrile and 1% acetic acid on an Inertsil ODS-2 column. The linear calibration ranges of sennosides A and B were 7.52-37.60 μg/ml (r=0.9998) and 8.32-49.92 μg/ml (r=0.9999), respectively. The recoveries of sennosides A and B were almost quantitative from the senna leaves and pods, respectively. The relative standard deviations of sennosides A and B for intraday and interday analyses were 0.24-1.72% and 0.23-1.71%, respectively. The contents of sennosides A and B in leaves and pods of Cassia angustifolia Vahl have been determined and the results were as follows: sennoside A 0.64, 1.46% and sennoside B 1.57, 2.30%. The contents of sennosides A and B in seven diet tea samples have been determined and the results were as follows: sennoside A 0.10-0.19% and sennoside B 0.16-0.28%."

A rapid high performance liquid chromatographic method was developed for determination of sennosides A and B in senna leaves, pods and diet tea. The determination was carried out by using a gradient elution with acetonitrile and 1% acetic acid on an Inertsil ODS-2 column. The linear calibration ranges of sennosides A and B were 7.52-37.60 µg/ml (r=0.9998) and 8.32-49.92 µg/ml (r=0.9999), respectively. The recoveries of sennosides A and B were almost quantitative from the senna leaves and pods, respectively. The relative standard deviations of sennosides A and B for intraday and interday analyses were 0.24-1.72% and 0.23-1.71%, respectively. The contents of sennosides A and B in leaves and pods of Cassia angustifolia Vahl have been determined and the results were as follows: sennoside A 0.64, 1.46% and sennoside B 1.57, 2.30%. The contents of sennosides A and B in seven diet tea samples have been determined and the results were as follows: sennoside A 0.10-0.19% and sennoside B 0.16-0.28%.