中藥材及血清中柚皮苷及柑果苷之高效層析定量   全文下載

HPLC Assays of Naringin and Hesperidin in Chinese Herbs and Serum

本研究建立高效液相層析法分析並定量中藥材枳實、枳殼和陳皮中以及人體血清中的柚皮?（naringin）及柑果?（hesperidin）兩成分。利用LiChrospher RP-18管柱為固定相，中藥材之水萃取物以水和氰甲烷之混合液為移動相進行梯度沖提（ 0-10 分鐘80 : 20，14-25分鐘63：37），ethylparaben為內標準；血清檢品則以水和氰甲烷之混合液為移動相進行等壓沖提（80 : 20），vanillin為內標準。兩種方法流速皆採1 ml／min，檢測波長皆以紫外光270 nm。同日內及異日間之測試結果顯示此二方法之精確度良好。回收率試驗顯示naringin和hesperidin皆可從血清或中藥材枳實、枳殼和陳皮中定量回收。

High performance liquid chromatographic methods for simultaneous determination of naringin and hesperidin in Chinese herbs and serum were established. By using a LiChrospher RP-18 column, the herbal extracts were separated by gradient elution with water-acetonitrile(0-10 min 80:20, 14-25 min 63:37) and the serum sample was separated by isocratic elution with water-acetonitrile(80:20) as the mobile phase. Both methods employed a flow rate of 1.0 ml/min with detection at 270 nm. Ethylparaben and vanillin were used as the internal standards in herbal extracts and in serum, respectively. The precision and accuracy of these methods were satisfactory for intraday and interday assays. The recoveries of naringin and hesperidin were almost quantitative from serum and from herbs including Fructus Aurantii Immaturus, Fructus Citri Immaturus Exsiccatus and Pericarpium Citri Chachiensis. 本研究建立高效液相層析法分析並定量中藥材枳實、枳殼和陳皮中以及人體血清中的柚皮苷（naringin）及柑果苷（hesperidin）兩成分。利用LiChrospher RP-18管柱為固定相，中藥材之水萃取物以水和氰甲烷之混合液為移動相進行梯度沖提（ 0-10 分鐘80 : 20，14-25分鐘63 : 37），ethylparaben為內標準；血清檢品則以水和氰甲烷之混合液為移動相進行等壓沖提（80 : 20），vanillin為內標準。兩種方法流速皆採1 ml／min，檢測波長皆以紫外光270 nm。同日內及異日間之測試結果顯示此二方法之精確度良好。回收率試驗顯示naringin和hesperidin皆可從血清或中藥材枳實、枳殼和陳皮中定量回收。