以動物細胞篩選抗癌症轉移之生物活性物質   全文下載

A Screening Program on Anti-metastasis from Microbial Metabolites

腫瘤的發生機制至今仍不清楚。腫瘤的可怕，在於其易於轉移，一旦發現時經常已有部分腫瘤細胞轉移至人體其他部位而不易以外科手術方法清除，造成癌症之再次發生。利用具有高度轉移力之細胞株，包括老鼠黑色素瘤細胞B16-F10、人類纖維肉瘤細胞HT-1080與低轉移力之細胞株──老鼠黑色素瘤B16-F1、人類胚胎正常肺纖維細胞MRC-5，作為抗癌症轉移物質之篩選模式，對台灣極端環境，如森林土、溫泉土、湖畔土、果園根圈之土壤中分離之100株本土絲狀真菌分離株之發酵萃取液進行篩選，應用美國國家癌症研究所（US National　Cancer Institute； 簡稱NCI）所發展的sulforhodamine （SRB） 染色方法進行細胞毒性試驗，篩選對高度轉移力之細胞株B16-F10、HT1080具有高生長抑制效應，而對低轉移力之B16-F1、MRC-5不具抑制作用或作用極低之生物活性物質，同時以人類肝癌細胞株Hep G2和人類血癌細胞株K562作為篩選物質對不同癌細胞專一性之對照，結果顯示某些分離株萃取物含具有抗癌症轉移作用之生物活性物質存在。本篇報告將介紹此生物活性物質篩選系統之篩選方法並利用此篩選系統所進行之篩選工作。 A malignant tumor is hard to eradicate by removing the mass surgically because the abnormal cells often metastasize. How to find and kill these metastatic cells is an important key to cure cancer. We have isolated over 100 filamentous fungi from soil of extreme environments in Taiwan, including forest, hot spring, lake and fruit tree rhizosphere in order to screen some biologically active compounds. Screening of anti-metastatic compounds, we used a set of highly metastatic cell lines, B16-F10 (mouse, melanoma) and HT-1 080 (human, fibrosarcoma) and cell lines with low metastatic ability, B16-F1 (mouse, melanoma) and MRC-5 (human, lung, normal diploid fibroblast). Cultures grown in microtiter plates were assayed by SRB (sulforho-damine B) method. The goal for this screening program is to isolate biologically active compounds that can inhibit the growth of highly metastatic cells such as B16-F10 and HT-1080 but little effect on low metastatic cells such as B10-F1 and MRC-5. Some strains' media showed significant differences in cytotoxicity between the highly metastatic cells and the low metastatic cells. These results suggest that some products in these media may contain metastasiss-pecific or cell-specific toxins."

A malignant tumor is hard to eradicate by removing the mass surgically because the abnormal cells often metastasize. How to find and kill these metastatic cells is an important key to cure cancer. We have isolated over 100 filamentous fungi from soil of extreme environments in Taiwan, including forest, hot spring, lake and fruit tree rhizosphere in order to screen some biologically active compounds. Screening of anti-metastatic compounds, we used a set of highly metastatic cell lines, B16-F10 (mouse, melanoma) and HT-1 080 (human, fibrosarcoma) and cell lines with low metastatic ability, B16-F1 (mouse, melanoma) and MRC-5 (human, lung, normal diploid fibroblast). Cultures grown in microtiter plates were assayed by SRB (sulforho-damine B) method. The goal for this screening program is to isolate biologically active compounds that can inhibit the growth of highly metastatic cells such as B16-F10 and HT-1080 but little effect on low metastatic cells such as B10-F1 and MRC-5. Some strains' media showed significant differences in cytotoxicity between the highly metastatic cells and the low metastatic cells. These results suggest that some products in these media may contain metastasiss-pecific or cell-specific toxins.