超臨界二氧化碳萃取紅檜木屑之成分分析研究：和水蒸氣蒸餾法及溶劑萃取法相比較   全文下載

Supercritical Carbon Dioxide Extraction of Red Cypress Sawdust: A Comparison of Solvent Extraction vs. Steam Distillation Methods

本實驗主要目的有二：第一是利用較先進且較符合環保要求的萃取方法－－超臨界二氧化碳萃取法（SFE-CO2），對紅檜木屑進行萃取。第二是配合其它不同的萃取方法，包括溶劑萃取法、水蒸氣蒸餾法以及頂空氣相法，來和SFE-CO2進行比較分析，這樣才能進一步了解SFE-CO2的萃取特性。紅檜木屑分別經各種不同的萃取方法萃取之後，注入氣相層析／質譜儀進行分析，再以資料庫搜尋系統（Library search）做比對，並比較其結果。結果顯示（1）對萃取檜木而言，二氧化碳在35°C、3000psi下，其極性是介於乙醚和水之間。（2）因為當使用nhexane來萃取時，所得之peak數目很少，且Abundance非常的低，所以顯示檜木之木屑中絕大部分是屬於極性物質。最後由於SFE-CO2 萃取沒有一個完美的經驗公式能夠計算任一物質的萃取率，所以針對每一物質萃取所得到的結果，均有其參考及利用之價值，尤其是目前以SFE-CO2萃取台灣本土植物的資料庫依然貧乏之際，更是值得大家用心去灌溉。

By using a supercritical carbon dioxide extraction (SFE-C02) technique, We extracted several fragrances from red cypress sawdust under a condition of 3500 psi and 35°C. The extracts were then analyzed by a gas chromatography/ mass spectrometry (GC/MS). After a Wiley mass spectrum library search, We identified most of the major peaks in the GC/MS total ion chromatogram, which are borneol, myrtenol, β-elemene, β-selinene, S-cadinene, and α-pinene, etc. for a total of 26 different compounds. In comparison with n-hexane extraction, ether extraction, steam distillation, and head space sampling techniques, we concluded that(l) the polarity of C02 under the condition of 3500 psi and 35°C is between ether and water; and(2) most of the fragrances in the cypress sawdust are polar compounds.