| 中文摘要 |
本研究開發並評估一簡易的氣相層析／質譜儀方法，供定量尿中 free codeine, free morphine, 6-acetylmorphine, total codeine, total morphine。抽取用之緩衝液以pH值為9.0時，其抽取回收率最高。抽取回收率，free codeine為98.3%，free morphine為76.7%，6-acetylmorphine為85.6%，三甲基矽化衍生物在七十二小時內均非常安定，適合其定量分析。nalorphine為內標準品，以SIM （Selected Ion Moni toring）方式定量。濃度在50至1000 ng／mL範為內均具有良好的線性關係，日內及日間精密度試驗顯示，codeine的變異係數為0.47 ~ 7.72%，morphine為0.31 ~ 8.09%，6-acetylmorphine為0.49 ~ 6.15%。" |
| 英文摘要 |
A simple yet effective GC/MS protocol was developed and evaluated for the determination of free codeine, free morphine, 6-acetylmorphine, total codeine and total morphine in urine. Optimal recovery was achieved with extraction conducted at pH 9.0. Trimethylsilyl-derivatives of these analytes and the internal standard (nalorphine) were found adequately stable for a 72- hour period. Quantitative determination of the analytes was performed by selective ion monitoring. Excellent linearity was observed over the 50-1000 ng/mL concentration range studied. The intraday and intraday precisions range from 0.47 to 7.72% for codeine, 0.31 to 8.09% for morphine and 0.49 to 6.15% for 6-acetylmorphine. The overall recoveries for codeine, morphine and 6-acetylmorphine were found to be 98.3%, 76.7% and 85.6% respectively. |
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