以高效能液相層析法分析魚肉中生物胺的含量   全文下載

Analysis of biogenic amines in fish muscles by High Performance Liquid Chromatography

本研究利用高效能液相層析法探討魚肉中七種生物胺之分析方法，其分析條件：逆相層析管柱以Inertsil 5 ODS-2 （250mm × 4.6mm，5μm），移動相是氰甲烷：甲醇：水 = 3：10：3 （V／V／V），流速1.2ml／min，檢測波長為254nm，能將不易分離的putrescine、cadaverine及spermine三種生物胺完全分離，由此使七種生物胺達到最大的分離效果，且於30分鐘內即可完成。七種生物胺濃度標準曲線都呈線性關係（r ＞0.90 以上）。偵測極限spermirle在0.6μg外，其餘六種生物胺則達0.3μg。添加於旗魚及秋刀魚的回收率大於89%以上。分析由超市中所購得的生魚肉中，鮪魚含有cadaverine，旗魚則測得cadaverine及spermine，草魚中僅spermine含量高於其它魚種；魟魚的putrescine、cadaverine及spermine含量均低於6.54mg／100g，土魠魚亦含此三種生物胺，但spermine含量較多13.10mg／100g；而秋刀魚及鮭魚則含較多種且多量的生物胺。旗魚的室溫貯藏試驗中發現，生鮮階段的旗魚只含單一生物胺spermine或少量的cadaverine，隨貯藏時間越長putrescine、cadaverine、histamine及spermidine等四種生物胺產生的量越多，產生速度以histamine ＞ cadaverine ＞ spermidine ＞ putrescine。

A HPLC procedure has been developed for the analysis of seven biogenic amines in fish. Inertsil 5 ODS-2 column with UV detection at 254nm and actonitrile-methanol-water (3:10:3, V/V/V) were used. The method is applicable to the quantification of amines with satisfactory sensitivity, selectivity and precision. It was found that tuna contains cadaverine ; grasscarp contains spermine ; swordfish contains cadaverine and spermine; barred spanish mackerel and rayfish each contain putrescine, cadaverine and spermine. Biogenic amines contents of pacific saury and salmon were higher than other fishes. In storage tests, fresh swordfish was shown to contain spermine and the longer the storage, the higher in the amount of purtrescine, cadaverine, histamine and spermidine. The order of producing rate of biogenic amine is histamine >cadaverine> spermidine> putrescine.