天麻藥材中指標成分Parishin, Parishin B及C之高效液相層析定量法之探討   全文下載

Determination of Parishin, Parishin, Band C in Gastrodiae Rhizoma by HPLC

本研究利用高效液相層析儀，分析天麻藥材中之高極性成分parishin、parishin B及C之含量，採用C18逆相層析管柱，移動相為甲醇－－0.1%磷酸溶液，利用線性梯度沖提，檢測波長為222 nm；得到良好分析結果。Parishin、parishin B及C三成分之線性迴歸方程式及相關係數（r）分別為：parishin （濃度80~1,200μg／ml）, Y= 433.82X－13.90 （r=0.9998），parishin B（濃度120~760μg／ml）, Y= 359.03X+55.98 （r=0.9831）及parishin C（濃度40~320μg／ml）, Y= 548.45X－60.66 （r=0.9995），均呈現良好線性關f系。添加回收率試驗結果，parishin為102.6 ± 3.3%，parishin B為102.8 ± 2.7%，parishin C為102.6 ± 2.9%，顯示準確性佳。三種指標成分之同日間及異日間試驗之相對標準偏差，分別為0.73～2.11%及0.67～3.56%，顯示高效液相層析法用於天麻指標成分之分析其結果良好。市售天麻藥材23種，利用上述高效液相層析法，定量分析各指標成分parishin、parishin B及C的含量百分比，結果parishin之含量則在0.37～1.50%之間，平均為0.96%；parishin B之含量在0.12～0.65%之間，平均為0.42%；parishin C之含量在0.08～0.27%之間，平均為0.16%。"

A rapid method by high performance liquid chromatography was developed for the simultaneous determination of three marker substances, parishin, parishins B and C, in Gastrodiae Rhizoma . This determination was carried out by using a gradient elution with methanol and 0.1% phosphoric acid on a Cosmosil 5C18-AR column. The linear calibration range of parishin, parishins Band C were 80～1200µg/ml (r=0.9998). 120～760µg/ml (r=0.9831) and 40～320µg/ml(r=0.9995), respectively. The recoveries of the markers were parishin: 102.6 ± 3.3%, parishin B: 102.8 ± 2.7% and parishin C: 102.6 ± 2.9%. The relative standard deviations of the three marker substances for intraday and interday analyses were 0.73～2.11% and 0.67～3.56%, respectively. The contents of the markers mentioned above in 23 crude drugs of Gastrodiae Rhizoma have been determined and the results were obtained as follows : parishin 0.37～1.50%, parishin B 0.12～0.65% and parishin C 0.08～0.27%. Therefore, HPLC is a useful technique to determine these selected markers.