大量快速篩選與新藥開發   全文下載

High Throughput Screening for New Drug Discovery

全球藥品市埸於一九九四年市值美金二千四百七十九億元到一九九九年市值預測為美金三千四百二十億元。在新藥開發研究方法上，大量快速篩選（High throughput screening, HTS）廣受注意。利用自動化機器、實驗室資訊管理系統，以及各種資源來大量快速篩選各種天然物（包括植物萃取、微生物代謝物）、庫存化學品、混式結合合成化學產品來尋找新藥。儀器設備、療效篩選目標之選擇、物料來源、樣品備製、初篩、複篩、有效物之分離純化、結構鑑定，皆為實施HTS之重要步驟。"

The world pharmaceutical market, which was valued at $247.9 billion in 1994, is forecasted to grow to $342 billion by 1999. High throughput screening(HTS) is attracting attention as a novel methodology for new drug discovery. HTS is expected to expand the scale from one thousand to one hundred thousand times the current level by utilizing robots, laboratory information management systems(LIMS), various sources to screen natural products(plant extracts, secondary microbial metabolites), peptide combinatorial libraries and combinatorial organic synthesis(COS) for new therapeutics. Instrumentation, target selection, source material, sample preparation, primary and secondary assays, isolation, purification and structure elucidation are all important for HTS.