以逆被動乳膠凝集方法偵測食品中產毒金黃色葡萄球菌及腸毒素   全文下載

Using Reversed Passive Latex Agglutination Method to Detect Enterotoxigenic Staphylococcus aureus and Enterotoxin in Foods

由市售食品（奶製品46件、肉製品76件及米製品74件，共計196件）及縣市衛生局送驗之食品中毒檢體（共計169件）中分離出之金黃色葡萄球菌，以逆被動乳膠凝集法（reversed passive latex agglutination, RPLA）測試產毒性及型別。市售食品金黃色葡萄球菌檢出率為16% （32／196）。以RPLA檢測具有產毒能力之菌株為56% （18／32），而毒素型別以A型最多（50%, 9／18）。食品中毒檢體中金黃色葡萄球菌之檢出率為32% （54／169），同樣以RPLA檢測，菌株其產毒能力者占78% （42／54），毒素型別亦以A型最多（81%, 34／42）。另對大腿、壽司、奶粉等食品分別以2.5、5、10及20 ng／g之腸毒素A （SEA）添加，探討最低檢出量（detection limit）。並以添加10、20及40 ng／g之SEA濃度，進行回收試驗。壽司及奶粉製品之最低檢出量可達5 ng／g，但SEA濃度若介於10-40 ng／g則回收率較佳。"

A total of 196 food samples including rice products, meat products and dairy products, and 169 food poisoning samples collected from local health bureaus were examined for Staphylococcus aureus. In addition, the enterotoxins produced by the isolates were determined using reversed passive latex agglutination (SET-RPLA). S. aureus was found in 16% (32/196) of food samples from markets and in 32% (54/169) of food poisoning samples. Furthermore, the most frequently found staphylococcal enterotoxin was type A which was detectecd in 50% (9/18) of the isolates from market food and in 81% (34/42) of the isolates from food poisoning samples. Enterotoxin A as low as 5 ng/g could be detected in rice and dairy products, and the recovery was better if SEA was present in foods at a concentration between 10-40ng/g.