苯巴比妥（PB）, 丁基羥基甲氧苯（BHA）與二丁基羥基甲苯（BHT）促進已起源作用之大鼠肝臟上皮細胞（INITIATED RAT EPITHELIAL CELL）之轉化（TRANSFORMATION）研究   全文下載

Studies on The Promotion of Transformation of Initiated Rat Liver Epithelial Cell by Phenobarbital, Butylated Hydroxyanisole and Butylated Hydroxytoluene

新生之大鼠（wistar），以2-乙醯胺基茀（2-acetylaminofluorene; 2-AAF）於生體內（in vivo）進行腫瘤起源作用（initiation），一星期後，肝藏經胰蛋白消化作用（trypsinization），後取得肝臟上皮細胞（liver epithelial cell），再進行生體外（in vitro）之促進作用（promotion）以測定苯巴比妥（PB），丁基羥基甲氧苯（BHA）與二丁基羥基甲苯（BHT）等之促進細胞轉化之活性。經起源作用之肝臟上皮細胞若於約20星期內未加以腫瘤促進作用之處理，則細胞可維持在不轉化（untransformation）之狀態。經生體內－－生體外（in vivo-in vitro）模式之研究結果，該三種物質在特定之濃度下不但明顯地影響已經起源之細胞生長與增殖活性（proliferative activity）亦明顯誘導肝臟上皮細胞轉化與惡性腫瘤（malignant tumor）標誌之γ-麩醯基轉胺?（γ-glutamyltranspeptidase），表面生長因子之鍵合（epidermal growth factor binding）及DNA量之增加，這些活性均出現明顯之劑量－－反應關係（dose-response manner）。

Newborne wistar rats were injected (i.p) with a single dose of 2-acetylaminofluorene (2-AAF) for in vivo model of initiation, one week later, the liver epithelial cells were obtained by a trypsinization of the pooled livers and used for in vitro model to evaluate the promotion of cell transformation by phenobarbital (PB), butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). In the absence of promoting treatment, the initiated liver epithelial cells remained untransformation at least during 20 weeks of culture. At the certain concentration, not only the growth and proliferative activity of the initiated liver epithelial cells were effected but also the marker of the transformation and malignant tumor such as the activity of γ-glutamyltranspeptidase, epidermal growth factor binding and DNA increasing were induced in a dose-response manner by in vivo-in vitro combination model.