以乳膠微粒凝聚法快達鑑定弧菌   全文下載

A Latex Agglutination Test for the Rapid Identification of Vibrios

前已知的弧菌（vibrios） 有30種（species）以上，其中9種可能引起人類疾病或食品中毒事件之發生。在製備腸炎弧菌（Vibrio parahaemolyticus）莢膜（capsu1e）抗體時，以13株混合並經加熱殺死之腸炎弧菌菌體（即莢膜抗原）免疫兔子得到之抗血清，發現這些抗體與大部分測試之弧菌屬細菌（Vibrio spp.）均有交叉反應。抗血清經過Aeromonas media CCRC 12211 吸附（absorption）後，被覆（coating）於乳膠微位上，當試劑與弧菌混合時，即產生凝聚反應，反應結果在1至2分鐘內可由肉眼判讀。此乳膠微粒凝聚法對弧菌（32種123株）大部分（99.2%）皆呈現正反應，對其他細菌（48種69株）則均為負反應。此種乳膠微粒凝聚法具有很高的敏感度（sensitivity）及專一性（specificity），可做為弧菌快速鑑定之用。

A latex agglutination test for the rapid identification of vibrios was developed. Anti-capsule antisera were raised in rabbits by immunizing the animals with mixed capsule antigens of 13 strains of Vibrio parahaemolyticus. Latex particles coated with the anti-capsule antibodies were used for the agglutination test of vibrios and other bacteria. Cross-reactions with some species of Aeromonas spp. were found, and could be eliminated by absorption of anti-capsule antibodies with Aeromonas media CCRC 12211. Latex coated with the absorbed and affinity-purified antibodies could agglutinate 99. 2% strains of vibrios (32 species, 123 isolates), and no cross-reactions were found with other bacteria (48 species, 69 isolates). Due to the high sensitivity and specificity, the latex agglutination test could be used for rapid identification of vibrios.