荊芥連翹湯中paeoniflorin, geniposide及glycyrrhizin成分之高效液相層析定量方法之研究   全文下載

Quantitative Analysis of Paeoniflorin, Geniposide and Glycyrrhizin in Jing-Jieh-lian-Otyau-Tong by High Performance liquid Chromatography

本研究應用高效液相層析法探討荊芥連翹湯中芍藥之芍藥?（paeoniflorin）、梔子之梔子?（geniposide）、甘草之甘草甜素（glycyrrhizin）等的分析方法，其分析條件為：（1）paeoniflorin、geniposide－－層析管柱：GL Sciences INC Inertsil 5 ODS-2, 250mm × 4.6 mm I.D. 5μm. 移動相：水.乙?（85：15），檢測波長：UV 254 nm.（2）glycyrrhizin－－層析管柱：Merck 50734 Lichrospher 100 RP-18 125mm × 4 mm I.D. 5μm. 移動相：1 %醋酸：乙?（65：35）。檢測波長：UV 254nm，各成分之檢量線顯示良好之線性關係，且paeoniflorin，geniposide及glycyrrhizin三者之添加回收率分別為100.0%、99.6%及99.9%，顯示有良好之精確性。另分析三家市售之濃縮製劑其paeoniflorin、geniposide及glycyrrhizin之最高最低差分別為2.22、4.75及4.62倍。為標準湯劑經減壓濃縮、冷凍乾燥及噴霧乾燥等方法處理上述三種成分含量幾不受影響。"

High Performance Liquid Chromatographic methods for determination of paeoniflorin in Pasoniae Radix, geniposide in Gardenia Radix and glycyrrhizin in Glycyrrhizae Radix were developed for the quality control of traditional Chinese medicinal prescriptions Jing-Jieh-LianChyau-Tong. Samples were extracted with 70% methanol by ultrasonic shaking for 30 minutes. The extract was separated by Inertsil 5 ODS-2 column with water-acetonitrile (85:15) as the mobile phase at a flow-rate of 1.0 ml/min. for paeoniflorin and geniposide, while for glycyrrhizin, it was separated by Lichrospher 100 RP-18 column with 1% acetic acid-acetonitrile (65:35) as the mobile phase at a flow-rate of 1.0 ml/min. The detecting wavelength was 254 nm with methylparaben and butylparaben used as the internal standards respectively. The calibration curves were linear in the range of 0.025-0.200 mg/ml for paeoniflorin; 0.02-0.10 mg/ml for geniposide and 0.016-0.150 mg/ml for glycyrrhizin. The recovery for each constituent from this prescription was 100.0% for paeoniflorin; 99.6% for geniposide and 99.9% for glycyrrhizin and the coefficient of variation was lower than 1.0%. The contents of these preparation constituents were not affected by treatment with concentration under reduced pressure, spray-drying or lyophilization respectively. The difference between the highest and lowest content of paeoniflorin, geniposide and glycyrrhizin were 4.2, 4.8 and 4.6-fold respectively.