市售尿液中安非他命類及鴉片類藥物簡易檢測套組之評估   全文下載

Evaluation of Five Commercial Amphetamines and Opiates Immunoassay Test Kits in Taiwan

本研究係針對常用之市售尿液中安非他命類及鴉片類藥物免疫學分析套組，如accuPINCH?、ONTRAK?，MACH IV(R)，I.D.Block?及BioSign?五種套組進行精密度及準確度之評估，以期選出適用之套組，供作例行尿液檢體分析的初步篩選試驗方法。精密度之評估係以一系列不同之甲基安非他命或嗎啡濃度分別進行測試，測試結果顯示，甲基安非他命部分除ONTRAK?於濃度1200 ng／ml時無法檢出外，其餘所有套組之最低檢量均低於其Cutoff值。準確度之評估則以經GC／MS確認之尿液檢體進行測試，測試結果顯示，甲基安非他命部分中除MACH IV(R)及ONTRAK?較差外，其餘套組尚可，而嗎啡部分中除MACH IV(R)較差外，其餘套組尚可。

Five conunonly used commercial amphetamines (AMs) and opiates (OPs) immunoassay test kits: HYCOR accuPINCH™, Drug Screening System (DSS) MACH TV (R)Screen™, Princeton BioMeditech (PBM) Biosign ™ and International Diagnostic Systems I. D. Block ™ were evaluated for their cutoff verification and assay performance with standardized and confirmed urine specimens. Cutoff verification was evaluated at several cutoffs for commercial AMs and OPs test kits. The assay performance was evaluated with authentic urine specimens confirmed by GC/MS. Results indicated that the real cutoffs for most AMs or OPs test kits were lower than those reported. accuPINCH™, AbuSign™ and I.D.Block™ were better than MACH IV(R)Screen™ and ONTRAK™ for AMs test kits; accuPINCH™, AbuSign™, I.D.Block™ and ONTRAK™ were better than MACH IV(R)Screen™ for OPs test kits.