養殖雌鳥維生素E攝食量與血漿及卵中維生素E含量及溶血之關係   全文下載

Influence of Dietary Vitamin E on the Content of Vitamin E in Blood Plasma, Ovary Tissue and on Hemolysis of Cultured Female Grey Mullet (Mugil cephalus)

為探討養殖雌烏攝食維生素E對其血漿及卵中維生素E含量與成熟時紅血球之影響，以已含90mg／kg α型維生素E之基本飼料，添加0、100、200 mg／kg α型維生素E投餵魚齡為一年之烏魚，飼養八個月。血漿維生素E濃度隨攝食維生素E量的增加而增加，隨生殖週期的接近而下降，至十一月將為最低，後上升。卵巢脂質中維生素E含量不因攝食90-290μmg／kg 維生素E而異，但隨生殖週期而變化，以十月最低296-376μg／g lipid，十一月最高602-696μg／g lipid，十二月再下降至408-453μg／g lipid，與血漿之維生素E濃度有互為消長的關係。烏魚的紅血球帶核，呈橢圓形，長軸大小介於9.42-9.58μm之間，短軸介於6.33-6.67μm之間，不因攝食維生素E量不同而明顯差異。紅血球經靜置8小時後，溶血率為7.55-7.71 %，溶血情形不因攝食維生素E量90-290mg／kg不同，而有明顯不同。但烏魚之體重及卵巢重則以攝食基本飼料者顯著低於攝食添加維生素至190-290mg／kg者（P）0.05） 。

Commercial diets containing a-tocopherol (90mg/kg) were supplemented with o, 100 and 200 mg/kg a-form vitamin E acetate respectively and fed to one-year-old (1 +) grey mullet (Mugil cephalus)for 12 months. Plasma vitamin E increased as dietary vitamin E increased, and showed seasonal changes. It decreased from May to November, then gradually increased until the following February. Vitamin E content in ovary was the lowest (296-376µg/g lipid) in October and the highest (602-694µg/g lipid) in November, then decreased to 408-453µg/g lipid in December. A negative relationship between vitamin E contents in plasma and in ovary was observed. Grey mullet erythrocytes appeared ellipsoidal in shape with a major axis of 9.24 to 9. 88µm and a minor axis of 6.33 to 6.67µm. No significant difference was found in shape and size of erythrocytes from grey mullet fed with diets of different vitamin E levels. Spontaneous hemolysis was 7.55-7.71%in an 8 h test, regardless of the dietary vitamin E intake of the fish. Body and ovary weights were significantly lower for mullet fed on commercial diets than fed on diets supplemented with vitamin E to 190 and 290 mg/kg.